Description

Cholestanol, 5α,6α-Epoxy-D7 is a deuterated derivative of 5α,6α-epoxycholestanol, a key sterol epoxide. This compound is of significant value as a stable isotope-labeled internal standard for precise quantitative analysis in mass spectrometry. It is primarily needed by pharmaceutical research laboratories, analytical chemistry facilities, and academic institutions engaged in lipidomics, metabolic pathway studies, and the development of diagnostic assays for sterol-related disorders.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of native sterol epoxides in complex biological matrices like plasma, serum, and tissue samples.
• Metabolic Tracing Studies: Used in deuterium-labeling experiments to investigate cholesterol oxidation pathways and metabolic flux.
• Biomarker Research: Facilitates the development and validation of analytical methods for oxysterols, which are potential biomarkers for various diseases.
• Pharmaceutical R&D: Supports drug discovery efforts targeting enzymes in the cholesterol metabolism pathway, such as cytochrome P450 enzymes.
• Quality Control Reference: Serves as a certified reference material (CRM) for ensuring the accuracy and precision of analytical methods in contract research organizations (CROs).
• Academic Research: Enables fundamental studies on the role of specific oxysterols in cell signaling, inflammation, and neurodegenerative diseases.

Basic Information

Item	Detail
Product Name	Cholestanol, 5α,6α-Epoxy-D7
CAS No.	127685-38-5
Molecular Formula	C27H39D7O2
Molecular Weight	~407.7 g/mol
Synonyms	5α,6α-Epoxycholestan-3β-ol-d7; (3β)-5α,6α-Epoxycholestan-3-ol-d7; 5α,6α-Epoxycholestanol-d7; Deuterated 5α,6α-Epoxycholestanol; Cholestane-3β,5α,6α-triol 5,6-anhydride-d7; 5,6α-Epoxy-5α-cholestan-3β-ol-d7; 3β-Hydroxy-5α,6α-epoxycholestane-d7
EINECS	Contact for details

Quality Control

Our Cholestanol, 5α,6α-Epoxy-D7 is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including NMR and High-Resolution Mass Spectrometry (HRMS), to confirm isotopic enrichment (≥98 atom % D) and chemical purity. Certificates of Analysis (COA) detailing all specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98%
Isotopic Purity (Atom % D)	≥98%
Chemical Purity (by HRMS)	≥95%
Solubility	Soluble in chloroform, DMSO; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.