Description

3-Nitrotetrazolium Blue Chloride is a vital tetrazolium salt derivative widely used as a redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to form a highly colored, insoluble formazan dye upon reduction, enabling the sensitive detection and quantification of dehydrogenase activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, particularly for cell viability testing (MTT assay alternatives), enzyme-linked immunosorbent assays (ELISAs), and histochemical staining.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in colorimetric assays (similar to MTT) to measure mitochondrial activity in live cells.
• Enzyme Activity Detection: Serves as an electron acceptor for detecting dehydrogenase and reductase enzymes in biochemical research and diagnostic kits.
• Histochemistry and Cytochemistry: Employed in tissue sections and cell smears to localize enzymatic activity, forming a visible formazan precipitate at reaction sites.
• Microbiological Testing: Applied in tests for bacterial identification and metabolic activity studies based on reduction capabilities.
• Biomedical Research: Utilized in studies of oxidative stress, cytotoxicity screening, and evaluation of drug effects on cellular metabolism.
• Diagnostic Reagent Formulation: A key component in the manufacture of reagents for clinical chemistry analyzers and point-of-care testing devices.

Basic Information

Product Name	3-Nitrotetrazolium Blue Chloride
CAS No.	127615-60-5
Molecular Formula	C40H30Cl2N10O6
Molecular Weight	817.64 g/mol
Synonyms	Nitro Blue Tetrazolium Chloride; NBT Chloride; NBT; Nitro BT; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride; Tetrazolium Blue Chloride; 3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazolium) dichloride; NTB Chloride
EINECS	Contact for details

Quality Control

Our 3-Nitrotetrazolium Blue Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with specifications for research and diagnostic use. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMF; slightly soluble in ethanol
Absorbance (λ max)	Characteristic peak in aqueous solution
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.