Description

L-Isoleucine-2-D1 is a stable isotope-labeled derivative of the essential amino acid L-Isoleucine, specifically deuterated at the 2-position. This compound is a critical tool for advanced research and analytical applications where precise molecular tracking is required. It is primarily utilized by scientists and manufacturers in the fields of pharmaceutical development, metabolic research, and analytical chemistry for use as an internal standard or tracer.

Application

• Mass Spectrometry Internal Standard: Used for the accurate quantification of L-Isoleucine in complex biological matrices via LC-MS/MS and GC-MS.
• Metabolic Pathway Tracing: Enables detailed studies of protein metabolism, amino acid turnover, and metabolic fluxes in biomedical and nutritional research.
• Pharmaceutical Research & Development: Serves as a key component in the development and validation of bioanalytical methods for pharmacokinetic and pharmacodynamic studies.
• Proteomics and Stable Isotope Labeling: Employed in quantitative proteomics (e.g., SILAC) to study protein expression and dynamics.
• NMR Spectroscopy: Acts as a deuterated probe for structural and dynamic studies of proteins and peptides.
• Quality Control Reference Material: Provides a certified reference standard for ensuring the accuracy and precision of analytical methods in quality control laboratories.

Basic Information

Product Name	L-Isoleucine-2-D1
CAS No.	127290-60-2
Molecular Formula	C6H12DNO2
Molecular Weight	132.18 g/mol
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic-2-d acid; (2S,3S)-2-Amino-3-methylpentanoic acid-2-d; L-Isoleucine-d1 (2-D); L-Isoleucine-2-d1; (2S,3S)-2-Azanyl-3-methyl-pentanoic-2-d acid; Deuterated L-Isoleucine; L-Ile-2-D1; Stable Isotope-Labeled L-Isoleucine
EINECS	Contact for details

Quality Control

Our L-Isoleucine-2-D1 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is characterized by advanced analytical techniques including NMR, MS, and HPLC to confirm isotopic enrichment, chemical identity, and purity. A comprehensive Certificate of Analysis (COA) detailing isotopic purity (e.g., D% > 98 atom %), chemical purity, and analytical results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Optical Rotation	[α]20/D: +39.5° to +41.5° (c=4 in 6N HCl)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.