Description

3'-o-Ethyl-N,n-Diisopropylphosphoramidite is a key phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of modified nucleosides, which is critical for developing advanced therapeutic and diagnostic nucleic acid sequences. It is primarily needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and life science sectors for producing antisense oligonucleotides, siRNAs, and primers.

Application

• Oligonucleotide Synthesis: As a critical building block in automated DNA/RNA synthesizers for the production of modified oligonucleotides.
• Therapeutic Nucleic Acid Development: For the synthesis of antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) used in gene silencing and targeted therapies.
• Diagnostic Probe Manufacturing: In creating labeled probes for PCR, sequencing, and microarray applications that require specific chemical modifications.
• Research & Development: For academic and industrial R&D focused on nucleic acid chemistry, structure-activity relationships, and novel biomolecule design.
• Primer and Adaptor Synthesis: Production of specialized primers and adaptors with terminal modifications for next-generation sequencing (NGS) and molecular cloning.
• Antisense Drug Manufacturing: A key raw material in the cGMP-compliant production pipeline for approved antisense drugs and clinical candidates.

Basic Information

Product Name	3'-o-Ethyl-N,n-Diisopropylphosphoramidite
CAS No.	125992-08-7
Molecular Formula	C14H31N2O3P
Molecular Weight	306.39 g/mol
Synonyms	3'-O-Ethyl-N,N-diisopropylphosphoramidite; 3'-O-Ethyl phosphoramidite; 5'-O-DMT-3'-O-ethyl-2'-deoxythymidine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (related protected form); 3'-Ethyl Phosphoramidite Monomer; 2-Cyanoethyl N,N-diisopropylchlorophosphoramidite, 3'-O-ethyl derivative; Amidite CEE; dT-3'-O-Ethyl Amidite; Oligonucleotide synthesis monomer 125992-08-7
EINECS	Contact for details

Quality Control

Our 3'-o-Ethyl-N,n-Diisopropylphosphoramidite is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and assay, NMR and IR for structural confirmation, and 31P NMR to verify phosphitylation efficiency. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research or cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at -20°C or below in a dedicated freezer. This material is hygroscopic (moisture-sensitive) and easily oxidized; exposure to air and moisture must be minimized to maintain reactivity and purity. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or clear colorless liquid
Identification (IR)	Conforms to structure
Identification (31P NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Assay	≥ 95.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.