Description

2-Cyanoethyl N,n- Diisopropylchlorophosphoramidite is a highly reactive phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the efficient, high-yield production of DNA and RNA sequences for research and therapeutic applications. This compound is essential for manufacturers and research institutions in the fields of molecular biology, genetic diagnostics, and the development of antisense oligonucleotides and siRNA therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the stepwise assembly of DNA and RNA strands on automated synthesizers.
• Antisense Oligonucleotide (ASO) Production: For the manufacture of therapeutic oligonucleotides designed to modulate gene expression.
• siRNA and miRNA Synthesis: A fundamental component in creating short interfering RNA and microRNA for gene silencing research and therapies.
• Primer and Probe Manufacturing: Used in the production of labeled and unlabeled oligonucleotides for PCR, sequencing, and diagnostic assays.
• Research & Development: For academic and industrial R&D labs exploring novel nucleic acid chemistries and applications.
• Pharmaceutical Intermediates: Serves as a vital intermediate in the cGMP production of oligonucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	2-Cyanoethyl N,n- Diisopropylchlorophosphoramidite
CAS No.	124482-92-4
Molecular Formula	C10H20ClN2OP
Molecular Weight	250.70 g/mol
Synonyms	2-Cyanoethyl N,N-Diisopropylchlorophosphoramidite; CE Phosphoramidite; Diisopropylamino-2-cyanoethoxyphosphine chloride; N,N-Diisopropylamino-2-cyanoethoxyphosphine chloride; Chloro(2-cyanoethoxy)diisopropylaminophosphine; Phosphoramidite monomer; DNA Synthesis Reagent; Amidite Reagent
EINECS	Contact for details

Quality Control

Our 2-Cyanoethyl N,n- Diisopropylchlorophosphoramidite is manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to relevant quality management standards to support both research and cGMP manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at 2-8°C in a dry, cool place. Due to its moisture-sensitive and easily oxidized nature, the container should only be opened in a dry, inert environment and resealed immediately after use.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid
Identification (IR)	Conforms
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.1%
P31 NMR	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.