Description

β-Hydroxyisovaleric Acid-D8 is a high-purity, deuterium-labeled isotopologue of β-hydroxyisovaleric acid, a key intermediate in leucine metabolism. This stable isotope-labeled compound is essential for precise quantitative analysis and metabolic pathway tracing in advanced research applications. It is primarily utilized by analytical laboratories and research institutions in the fields of metabolomics, clinical diagnostics, and pharmaceutical development.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, non-interfering internal standard for the accurate quantification of endogenous β-hydroxyisovaleric acid in complex biological matrices like plasma, urine, and tissue samples.
• Metabolomics Research: Used in stable isotope dilution mass spectrometry (SID-MS) to study disorders of branched-chain amino acid metabolism, such as maple syrup urine disease (MSUD).
• Clinical Diagnostics: Enables the development and validation of highly specific and sensitive assays for diagnosing inborn errors of metabolism.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Facilitates tracer studies to investigate drug mechanisms and their impact on specific metabolic pathways.
• Biochemical Pathway Analysis: Acts as a tracer to elucidate the flux through the leucine degradation pathway in cellular and animal models.
• Reference Material: Provides a certified reference material for quality control and method calibration in analytical laboratories.

Basic Information

Product Name	β-Hydroxyisovaleric Acid-D8
CAS No.	123743-99-7
Molecular Formula	C5H2D8O3
Molecular Weight	136.19 g/mol
Synonyms	3-Hydroxy-3-methylbutanoic acid-d8; 3-Hydroxyisovaleric acid-d8; β-Hydroxy-β-methylbutyric acid-d8; HMB-d8; 3-Methyl-3-hydroxybutyric acid-d8; (RS)-3-Hydroxy-3-methylbutanoic acid-d8; 3-Hydroxy-3-methylbutyric acid-d8; Deuterated β-hydroxyisovaleric acid
EINECS	Contact for details

Quality Control

Every batch of β-Hydroxyisovaleric Acid-D8 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We guarantee high chemical and isotopic purity, verified by advanced techniques including NMR (1H, 13C) and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, isotopic enrichment, and solvent content to ensure consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere to maintain optimal stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.