Description

Cholestane-3,3-D2 is a deuterium-labeled analog of cholestane, a fundamental steroidal hydrocarbon. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring analytical accuracy and precision. It is primarily required by research institutions, pharmaceutical companies, and contract research organizations (CROs) engaged in lipidomics, metabolic studies, and the development of analytical methods for sterol and steroid analysis.

Application

• Internal Standard for Mass Spectrometry (MS): Critical for the accurate quantification of cholesterol and related sterols in biological matrices via GC-MS or LC-MS/MS.
• Metabolic Pathway Tracing: Used in deuterium-labeling studies to investigate cholesterol biosynthesis, absorption, and turnover.
• Pharmaceutical Research & Development: Supports drug discovery and development programs targeting lipid metabolism, such as for hypercholesterolemia.
• Clinical Diagnostics: Enables precise measurement of sterol biomarkers in patient samples for research into metabolic disorders.
• Biochemical Research: Serves as a reference compound in the study of membrane biophysics and lipid raft composition.
• Food & Nutrition Science: Applied in research analyzing sterol content and bioavailability in food products and dietary studies.

Basic Information

Product Name	Cholestane-3,3-D2
CAS No.	122241-86-5
Molecular Formula	C₂₇H₄₆D₂
Molecular Weight	374.67 g/mol
Synonyms	5α-Cholestane-3,3-D2; 3,3-Dideutero-5α-cholestane; Cholestane-d2 (3,3); (3α,5α)-3,3-Dideuterocholestane; 5α-Cholestane-3β,3β-D2; NSC 18186-d2; Deuterated Cholestane; Stable Isotope Labeled Cholestane
EINECS	Contact for details

Quality Control

Our Cholestane-3,3-D2 is manufactured under strict quality systems to ensure high chemical and isotopic purity, which is critical for its role as an analytical standard. Each batch is characterized by NMR, MS, and HPLC to confirm identity, deuterium incorporation, and chemical purity. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (e.g., D% or atom % D), chemical purity, and analytical methods is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing under an inert atmosphere at -20°C to ensure optimal stability.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Deuterium Content	≥ 99.0% at labeled positions
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.