Description

Dimethyl N,n-Diisopropylphosphoramidite (CAS NO 122194-07-4) is a highly reactive organophosphorus compound primarily used as a key phosphitylating reagent in oligonucleotide synthesis. Its core value lies in enabling the efficient and controlled formation of phosphite triester intermediates, which are critical for constructing the phosphate backbone of DNA and RNA strands. This chemical is essential for manufacturers and research institutions engaged in the production of primers, probes, antisense oligonucleotides, and other nucleic acid-based therapeutics and diagnostics.

Application

• Oligonucleotide Synthesis: A fundamental phosphitylating agent in solid-phase phosphoramidite chemistry for automated DNA/RNA synthesizers.
• Pharmaceutical Intermediates: Serves as a critical building block in the synthesis of nucleotide-based active pharmaceutical ingredients (APIs) and prodrugs.
• Research & Development: Used in academic and industrial labs for the custom synthesis of modified oligonucleotides for genetic research, diagnostics, and drug discovery.
• Antisense Therapy Production: Key reagent in the manufacturing of antisense oligonucleotides designed to target specific RNA sequences for therapeutic purposes.
• siRNA and miRNA Synthesis: Employed in the production of small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing applications.
• Diagnostic Probe Manufacture: Used to create labeled DNA and RNA probes for various diagnostic assays, including PCR and fluorescence in situ hybridization (FISH).

Basic Information

Product Name	Dimethyl N,n-Diisopropylphosphoramidite
CAS No.	122194-07-4
Molecular Formula	C8H20NO2P
Molecular Weight	193.22 g/mol
Synonyms	Diisopropylphosphoramidous Acid Dimethyl Ester; N,N-Diisopropylphosphoramidous Acid Dimethyl Ester; Dimethyl Diisopropylphosphoramidite; Phosphoramidous Acid, N,N-Diisopropyl-, Dimethyl Ester; (Dimethoxyphosphanyl)-N,N-diisopropylamine; Diisopropylamino-dimethylphosphine
EINECS	Contact for details

Quality Control

Our Dimethyl N,n-Diisopropylphosphoramidite is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR, HPLC, and Karl Fischer titration, to ensure high purity and consistency suitable for sensitive oligonucleotide synthesis applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to guarantee traceability and compliance with your internal quality standards.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic and easily oxidized nature, this product must be stored under an inert atmosphere (argon or nitrogen) in a cool, dry place. Recommended storage temperature is 2-8°C for long-term stability. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.