Description

L-Phenyl-D5-Alanine-n-T-Boc is a stable isotope-labeled derivative of the essential amino acid phenylalanine, specifically deuterated at five positions and protected with a tert-butoxycarbonyl (Boc) group. This high-purity compound is critical for ensuring accuracy and reliability in advanced analytical research, particularly in quantitative mass spectrometry. It is primarily utilized by research scientists and process developers in the pharmaceutical, biotechnology, and academic sectors for applications requiring precise internal standards and metabolic pathway tracing.

Application

• Quantitative Mass Spectrometry (MS) Internal Standard: Serves as a critical reference compound for the accurate quantification of phenylalanine and related metabolites in complex biological matrices like plasma, urine, and tissue samples.
• Metabolic Flux Analysis & Proteomics Research: Used in tracer studies to investigate amino acid metabolism, protein synthesis rates, and cellular pathways in both in vitro and in vivo systems.
• Pharmaceutical Drug Development & Pharmacokinetics (PK/PD): Enables precise measurement of drug candidates and their metabolites in preclinical and clinical studies, supporting ADME (Absorption, Distribution, Metabolism, Excretion) profiling.
• Biomarker Discovery & Clinical Diagnostics: Facilitates the development of robust LC-MS/MS assays for detecting and quantifying disease-specific biomarkers related to amino acid disorders.
• Synthesis of Labeled Peptides & Proteins: Acts as a key building block for the solid-phase or solution-phase synthesis of deuterium-labeled peptides used in proteomics and structural biology.
• Nutritional and Sports Science Research: Applied in studies examining protein metabolism, muscle synthesis, and the metabolic effects of dietary interventions.

Basic Information

Product Name	L-Phenyl-D5-Alanine-n-T-Boc
CAS No.	121695-40-7
Molecular Formula	C₁₄H₁₄D₅NO₄
Molecular Weight	~ 280.36 g/mol
Synonyms	(2S)-2-[(tert-Butoxycarbonyl)amino]-3-(2,3,4,5,6-pentadeuteriophenyl)propanoic acid; N-Boc-L-phenylalanine-d5; Boc-Phe(ring-d5)-OH; tert-Butyl (2S)-2-amino-3-(2,3,4,5,6-pentadeuteriophenyl)propanoate; L-Phenylalanine-2,3,4,5,6-d5, N-Boc derivative; N-(tert-Butoxycarbonyl)-L-phenylalanine-d5; Boc-L-Phe(ring-d5)-OH; Deuterated Boc-Phenylalanine
EINECS	Contact for details

Quality Control

Our L-Phenyl-D5-Alanine-n-T-Boc is manufactured under strict quality systems to ensure the high isotopic purity and chemical integrity required for sensitive analytical applications. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % D), chemical purity, and specific tests is provided with every shipment to guarantee traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This compound is hygroscopic (moisture-sensitive); therefore, containers must be kept sealed under inert atmosphere (e.g., nitrogen or argon) after opening to prevent degradation and maintain isotopic integrity. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Optical Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.