Description

Citric-2,4-13C2 Acid is a stable isotope-labeled analog of citric acid, specifically enriched with carbon-13 at the 2 and 4 positions. This precise labeling makes it an indispensable internal standard and tracer for advanced metabolic and biochemical research using techniques like NMR spectroscopy and mass spectrometry. It is primarily required by analytical laboratories, pharmaceutical R&D departments, and academic institutions conducting studies in metabolomics, flux analysis, and drug metabolism.

Application

• As a quantitative internal standard for the accurate measurement of endogenous citric acid/citrate levels in biological samples via LC-MS or GC-MS.
• For metabolic flux analysis (MFA) to trace and quantify carbon flow through the citric acid (Krebs) cycle in cellular systems.
• In NMR spectroscopy studies to investigate enzyme mechanisms, metabolic pathways, and structural dynamics due to the distinct 13C signal.
• Use in pharmaceutical research for studying drug metabolism and pharmacokinetics (DMPK) where citric acid cycle intermediates are involved.
• As a critical reagent in the development and validation of clinical diagnostic assays for inborn errors of metabolism.
• For nutritional and isotopic tracer studies in animal and human research to understand energy metabolism.

Basic Information

Item	Detail
Product Name	Citric-2,4-13C2 Acid
CAS No.	121633-50-9
Molecular Formula	C613C2H8O7
Molecular Weight	~194.11 g/mol (based on 13C enrichment)
Synonyms	2,4-13C2-Citric Acid; [13C2]-Citric Acid; Citric Acid-2,4-13C2; (1,3-13C2)Propane-1,2,3-tricarboxylic Acid; 2-Hydroxy-1,2,3-propanetricarboxylic-2,4-13C2 Acid; 3-Carboxy-3-hydroxypentanedioic-2,4-13C2 Acid; Isotopically Labeled Citric Acid; 13C Labeled Citrate
EINECS	Contact for details

Quality Control

Our Citric-2,4-13C2 Acid is manufactured under controlled conditions to ensure exceptional isotopic and chemical purity. Each batch undergoes rigorous quality testing, including analysis by NMR and mass spectrometry, to confirm enrichment level, isotopic purity, and chemical purity. Certificates of Analysis (COA) detailing all specifications are provided to guarantee consistency and reliability for your sensitive research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect stability and weighing accuracy.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0% (chemical purity)
Isotopic Enrichment	≥99 atom % 13C (at positions 2 & 4)
Isotopic Purity (NMR/MS)	≥98%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.