Description

5'-o-Dmt-N6-Bz-Ppa is a key protected nucleoside phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity during automated DNA/RNA synthesis. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing antisense oligonucleotides, siRNAs, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite) for the automated solid-phase synthesis of DNA and RNA strands.
• Therapeutic Nucleic Acid Development: Crucial for the research and production of antisense oligonucleotides, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Manufacturing: Used to synthesize labeled probes for PCR, qPCR, FISH, and microarray applications.
• Research Grade Oligos: Serves as a high-purity monomer for synthesizing custom primers and research oligonucleotides in academic and industrial labs.
• Next-Generation Sequencing (NGS): Employed in the synthesis of oligonucleotide libraries and adapters for sequencing platforms.
• CRISPR Guide RNA Synthesis: A component in the chemical synthesis of guide RNAs (gRNAs) for gene editing applications.

Basic Information

Product Name	5'-o-Dmt-N6-Bz-Ppa
CAS No.	119803-27-9
Molecular Formula	C46H51N6O7P
Molecular Weight	830.92 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-dA(Bz)-CE Phosphoramidite; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dA(Bz) Phosphoramidite; 2'-Deoxyadenosine, N6-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Protected Deoxyadenosine Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-o-Dmt-N6-Bz-Ppa is manufactured under strict quality control protocols to ensure it meets the high-purity standards required for sensitive oligonucleotide synthesis. Each batch is tested using advanced analytical techniques including HPLC and 31P NMR to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with lot-specific data are provided to guarantee reproducible performance and supply chain traceability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Moisture Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.