Description

Dmt-2′o-Tbdms-Ru Phosphoramidite is a high-purity, specialty phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for introducing a specific, protected ribonucleoside building block into RNA sequences, enabling the production of research-grade and therapeutic oligonucleotides. It is an essential raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on RNA-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated synthesis of RNA strands.
• Therapeutic RNA Production: Manufacturing of siRNA, antisense oligonucleotides, and mRNA for therapeutic applications.
• Research Reagent: Synthesis of custom RNA probes, primers, and standards for molecular biology and biochemistry research.
• Diagnostic Probe Synthesis: Production of labeled RNA probes for diagnostic assays and genomic testing.
• Antisense Technology: Building block for creating antisense RNA molecules used in gene function studies and drug discovery.
• CRISPR Guide RNA Synthesis: Component in the synthesis of guide RNAs (gRNAs) for CRISPR-Cas9 gene editing systems.

Basic Information

Product Name	Dmt-2′o-Tbdms-Ru Phosphoramidite
CAS No.	118362-03-1
Molecular Formula	C41H51N4O8PSi
Molecular Weight	787.0 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)uridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-TBDMS-5'-O-DMT-Uridine Phosphoramidite; DMT-2'-O-TBDMS-Uridine Phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-O-TBDMS-uridine phosphoramidite; RNA Phosphoramidite Monomer (U); U Phosphoramidite (2'-O-TBDMS)
EINECS	Contact for details

Quality Control

Every batch of Dmt-2′o-Tbdms-Ru Phosphoramidite is manufactured and tested under strict quality management systems. We ensure high purity and consistency through advanced analytical techniques including HPLC, 31P NMR, and 1H NMR. Certificates of Analysis (COA) with detailed chromatographic data are provided for each lot, supporting compliance with cGMP guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and should be handled in a controlled, dry atmosphere to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.