Description

4-Chloro-3-Indoxyl-1,3-Diacetate is a high-purity chromogenic substrate and key biochemical intermediate. This compound matters for its critical role in enzymatic detection systems, particularly for β-galactosidase and related hydrolases, enabling sensitive colorimetric and fluorometric assays. It is essential for researchers and manufacturers in the life sciences, diagnostic kit production, and pharmaceutical development who require reliable, high-quality substrates for molecular biology and histochemistry applications.

Application

• Enzymatic Substrate for β-Galactosidase: Primary use as a chromogenic agent in molecular biology for lacZ gene reporter assays and bacterial colony screening.
• Diagnostic Reagent Manufacturing: Key raw material in the production of diagnostic kits for clinical microbiology and genetic testing.
• Histochemistry and Cytochemistry: Used in tissue staining to localize enzyme activity, particularly in neuroscience and developmental biology research.
• Biochemical Research: Employed in studies of glycosidase enzyme kinetics, inhibition, and mechanism of action.
• Pharmaceutical Intermediate: Serves as a specialized building block in the synthesis of more complex indole-based pharmaceutical compounds.
• Microbial Detection: Applied in food safety and water quality testing for the specific detection of E. coli and other β-galactosidase-producing organisms.

Basic Information

Product Name	4-Chloro-3-Indoxyl-1,3-Diacetate
CAS No.	109014-54-2
Molecular Formula	C13H12ClNO4
Molecular Weight	281.69 g/mol
Synonyms	4-Chloro-3-indolyl diacetate; 5-Chloro-3-indoxyl diacetate; 4-Chloro-3-indoxyl acetate; 4-Cl-3-I-1,3-DA; 4-Chloroindoxyl diacetate; 3-(Acetyloxy)-4-chloro-1H-indol-1-yl acetate; 4-Chloro-3-indolol 1,3-diacetate
EINECS	Contact for details

Quality Control

Our 4-Chloro-3-Indoxyl-1,3-Diacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and stringent control of moisture and related substances. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This product is hygroscopic (moisture-sensitive). Allow the container to reach ambient temperature before opening to minimize moisture condensation. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 0.5%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.