Description

Phenolphthalein Monophosphate Disodium Salt is a key chromogenic substrate used in enzymatic assays for the detection of alkaline phosphatase activity. Its high sensitivity and specificity make it an indispensable reagent for precise biochemical analysis and diagnostic applications. This compound is primarily utilized by manufacturers and researchers in the in-vitro diagnostics (IVD), life science research, and pharmaceutical quality control sectors.

Application

• Clinical Diagnostics: As a critical substrate in enzymatic immunoassays (EIA/ELISA) for the detection of alkaline phosphatase-labeled antibodies or antigens.
• Biochemical Research: Used in laboratory research for studying enzyme kinetics, inhibition, and activity of alkaline phosphatase in various biological samples.
• IVD Kit Manufacturing: A core raw material for the production of diagnostic test kits, including automated clinical chemistry analyzers and point-of-care testing devices.
• Pharmaceutical QC: Employed in quality control testing within biopharmaceutical production to monitor and validate purification processes.
• Histology & Cytochemistry: Acts as a substrate in staining procedures for the localization of alkaline phosphatase activity in tissue sections and cell cultures.
• Food Safety Testing: Potential use in enzymatic methods for detecting bacterial contamination, as certain bacteria produce alkaline phosphatase.

Basic Information

Product Name	Phenolphthalein Monophosphate Disodium Salt
CAS No.	108321-15-9
Molecular Formula	C20H15O8PNa2
Molecular Weight	460.28 g/mol
Synonyms	Phenolphthalein Diphosphate Disodium Salt; Phenolphthalein Phosphate Disodium Salt; Disodium Phenolphthalein Phosphate; 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone Monophosphate Disodium Salt; PPP; PNPP (commonly confused with p-Nitrophenyl Phosphate); Alkaline Phosphatase Substrate; Diagnostic Reagent Grade Phenolphthalein Phosphate
EINECS	Contact for details

Quality Control

Our Phenolphthalein Monophosphate Disodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the stringent requirements for diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	≥ 95.0% (as substrate for alkaline phosphatase)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, buffer solutions
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.