Description

L-Leucine-2,3,3,4,5,5,5,6,6,6-D10 is a stable isotope-labeled analog of the essential amino acid L-leucine, where ten hydrogen atoms are replaced by deuterium. This high level of deuteration makes it an invaluable internal standard for quantitative mass spectrometry, ensuring unparalleled accuracy and reproducibility in analytical measurements. It is primarily required by research institutions, pharmaceutical companies, and CROs engaged in advanced metabolomics, proteomics, pharmacokinetic studies, and the development of novel diagnostic assays.

Application

• Quantitative Mass Spectrometry (MS): Serves as a critical internal standard for the precise quantification of endogenous L-leucine and related metabolites in complex biological matrices like plasma, urine, and tissue homogenates.
• Metabolomics & Biomarker Discovery: Enables accurate tracking of leucine metabolism pathways in studies of metabolic disorders, cancer research, and nutritional science.
• Pharmacokinetics (PK) & Drug Metabolism Studies: Used in stable isotope dilution assays to determine the absorption, distribution, metabolism, and excretion (ADME) profiles of pharmaceutical compounds.
• Proteomics & Protein Turnover Analysis: Incorporated into SILAC (Stable Isotope Labeling by Amino acids in Cell culture) or other pulse-chase experiments to measure dynamic protein synthesis and degradation rates.
• Clinical Diagnostics & Newborn Screening: Employed in LC-MS/MS methods for the reliable diagnosis of inborn errors of metabolism such as maple syrup urine disease (MSUD).
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Acts as a deuterated probe for structural and dynamic studies of proteins and peptides without signal interference from hydrogen.
• Nutritional Research: Facilitates the precise measurement of leucine flux and utilization in studies on muscle protein synthesis, sports science, and dietary interventions.

Basic Information

Product Name	L-Leucine-2,3,3,4,5,5,5,6,6,6-D10
CAS No.	106972-44-5
Molecular Formula	C6D10H3NO2
Molecular Weight	137.21 g/mol (for D10 isotopologue)
Synonyms	(2S)-2-Amino-4-methylpentanoic-2,3,3,4,5,5,5,6,6,6-d10 acid; L-Leucine-d10; Deuterated L-Leucine; Leucine-2,3,3,4,5,5,5,6,6,6-D10; (S)-2-Amino-4-methylpentanoic-d10 acid; [2,3,3,4,5,5,5,6,6,6-2H10]L-Leucine; Leucine (Perdeuterated, specific positions); Stable Isotope-Labeled Leucine
EINECS	Contact for details

Quality Control

Our L-Leucine-2,3,3,4,5,5,5,6,6,6-D10 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % D), chemical purity, and chiral purity. Our quality commitment aligns with the standards expected for research and diagnostic use, and we support compliance with ISO 17025 and GMP where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, cool place. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % D)	≥ 98.0% (by MS)
Chiral Purity (Optical Rotation)	[α]D20: +14.5° to +16.5° (c=2 in 6N HCl)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.