Description

L-Phenyl-D5-Alanine-2,3,3-D3-n-T-Boc is a stable isotope-labeled derivative of the essential amino acid phenylalanine, featuring deuterium atoms at specific positions and a tert-butoxycarbonyl (Boc) protecting group on the amine. This compound is critical for ensuring accuracy and reliability in quantitative mass spectrometry-based analyses, where it serves as an internal standard. It is primarily required by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for applications in drug metabolism and pharmacokinetics (DMPK) studies, proteomics, and metabolic research.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for accurate measurement of phenylalanine and related compounds in complex biological matrices.
• Drug Metabolism Studies (DMPK): Essential for tracing and quantifying drug metabolites in preclinical and clinical research to understand absorption, distribution, metabolism, and excretion (ADME) profiles.
• Proteomics and Metabolic Research: Employed in stable isotope labeling by amino acids in cell culture (SILAC) and other techniques to study protein dynamics, turnover, and metabolic pathways.
• Biomarker Discovery and Validation: Facilitates the precise quantification of potential disease biomarkers in plasma, serum, urine, and tissue samples.
• Pharmaceutical Quality Control: Supports analytical method development and validation for the assay of amino acid-based active pharmaceutical ingredients (APIs).
• Nutritional Science Research: Used in studies investigating amino acid metabolism, protein synthesis, and nutritional status.

Basic Information

Product Name	L-Phenyl-D5-Alanine-2,3,3-D3-n-T-Boc
CAS No.	106881-07-6
Molecular Formula	C₁₄H₁₃D₈NO₄
Molecular Weight	284.38 g/mol
Synonyms	(2S)-2-[(tert-Butoxycarbonyl)amino]-3-(2,3,4,5,6-pentadeuteriophenyl)(2,3,3-2H3)propanoic Acid; Boc-Phe(D5,2,3,3-D3)-OH; N-Boc-L-Phenylalanine-D5-2,3,3-D3; tert-Butyl (2S)-2-amino-3-(2,3,4,5,6-pentadeuteriophenyl)(2,3,3-2H3)propanoate; N-(tert-Butoxycarbonyl)-L-phenylalanine-D5-2,3,3-D3; Boc-L-Phe(D5,2,3,3-D3); L-Phenylalanine-D5-2,3,3-D3, N-Boc-; Stable Isotope Labeled Boc-Phenylalanine
EINECS	Contact for details

Quality Control

Our L-Phenyl-D5-Alanine-2,3,3-D3-n-T-Boc is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm identity, isotopic enrichment, and chemical purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.