Description

4-Hydroxy Coumarin-D4 is a stable isotope-labeled analog of the bioactive compound 4-hydroxycoumarin, where four hydrogen atoms are replaced by deuterium. This deuteration is critical for enhancing the precision and reliability of analytical methods, particularly in mass spectrometry. It serves as an essential internal standard for researchers and quality control professionals in the pharmaceutical and analytical chemistry sectors who require accurate quantification of the parent compound in complex biological or chemical matrices.

Application

• Primary use as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the quantification of 4-hydroxycoumarin.
• Critical component in pharmacokinetic and metabolic studies to track the absorption, distribution, metabolism, and excretion of coumarin-based drugs.
• Enables high-accuracy analysis in bioanalytical research and clinical diagnostics, minimizing matrix effects and instrument variability.
• Used in the development and validation of regulatory-grade analytical methods for pharmaceutical quality control and forensic analysis.
• Facilitates research into the biological activity, environmental fate, and degradation pathways of coumarin derivatives.

Basic Information

Product Name	4-Hydroxy Coumarin-D4
CAS No.	106754-18-1
Molecular Formula	C9H4D4O3
Molecular Weight	~ 180.18 g/mol
Synonyms	4-Hydroxy-2H-1-benzopyran-2-one-d4; 4-Hydroxy-2H-chromen-2-one-d4; 4-Hydroxycoumarin-d4; 2H-1-Benzopyran-2-one, 4-hydroxy-, d4-; Deuteronated 4-Hydroxycoumarin; [D4]-4-Hydroxycoumarin; 4-HC-d4; Isotopically Labeled 4-Hydroxycoumarin
EINECS	Contact for details

Quality Control

Our 4-Hydroxy Coumarin-D4 is manufactured under strict quality management systems to ensure the highest isotopic purity and chemical integrity. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR and HPLC-MS, to confirm deuteration level and chemical purity. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98% D), chemical purity, and analytical results is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuteration)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.