Description

Cytosine-5,6-D2 is a stable isotope-labeled analog of the nucleobase cytosine, specifically deuterated at the 5 and 6 positions. This deuteration is critical for applications requiring non-radioactive isotopic tracing, enhancing the precision of analytical studies in metabolic and pharmacokinetic research. It is an essential building block for researchers and manufacturers in the fields of pharmaceutical development, advanced NMR spectroscopy, and mass spectrometry.

Application

• Pharmaceutical Research & Development: Used as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of cytosine and its derivatives in biological matrices.
• Metabolic Pathway Tracing: Serves as a stable isotopic tracer to study the metabolism, absorption, and distribution of nucleosides and related drugs in vivo.
• NMR Spectroscopy: Provides a valuable probe for structural and dynamic studies of nucleic acids and their interactions with proteins or small molecules, leveraging the distinct NMR properties of deuterium.
• Synthesis of Labeled Compounds: Acts as a key precursor for the synthesis of more complex deuterated nucleosides, nucleotides, and oligonucleotides.
• Biochemical Assays: Employed in enzyme kinetics studies to investigate the mechanisms of enzymes involved in nucleotide synthesis and catabolism.

Basic Information

Product Name	Cytosine-5,6-D2
CAS No.	106391-24-6
Molecular Formula	C4H3D2N3O
Molecular Weight	113.11 g/mol
Synonyms	4-Amino-2(1H)-pyrimidinone-5,6-d2; 2-Oxo-4-aminopyrimidine-5,6-d2; 5,6-Dideuteriocytosine; Cytosine-d2; [5,6-D2]Cytosine; 4-Amino-1H-pyrimidin-2-one-5,6-d2
EINECS	Contact for details

Quality Control

Our Cytosine-5,6-D2 is manufactured under strict quality control protocols to ensure high isotopic purity and chemical integrity. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D%), chemical purity, and identification. We adhere to cGMP principles for research-grade materials, ensuring reliable and reproducible performance in sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (D%)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.