Description

Bis(2-Cyanoethyl)-N,n-Diisopropyl Phosphoramidite is a high-purity phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. This compound is essential for the production of DNA and RNA sequences used in research, diagnostics, and therapeutic development. It is primarily required by manufacturers in the pharmaceutical, biotechnology, and life science research sectors for applications in antisense therapy, PCR primers, and gene synthesis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Research Reagent: Synthesis of custom primers, probes, and gene fragments for molecular biology and genetic research.
• Diagnostic Kit Manufacturing: Production of labeled probes and components for diagnostic assays and kits.
• Next-Generation Sequencing (NGS): Synthesis of oligonucleotide libraries and adapters used in sequencing platforms.
• Synthetic Biology: Construction of synthetic genes and genetic circuits.

Basic Information

Product Name	Bis(2-Cyanoethyl)-N,n-Diisopropyl Phosphoramidite
CAS No.	102690-88-0
Molecular Formula	C13H24N3O2P
Molecular Weight	285.33 g/mol
Synonyms	N,N-Diisopropyl-bis(2-cyanoethyl)phosphoramidite; Diisopropylamino-bis(2-cyanoethoxy)phosphine; 2-Cyanoethyl N,N-Diisopropylphosphoramidite (dimer form); CE Phosphoramidite; DNA Synthesis Phosphoramidite Monomer; Phosphoramidite CE; β-Cyanoethyl N,N-diisopropylphosphoramidite
EINECS	Contact for details

Quality Control

Our Bis(2-Cyanoethyl)-N,n-Diisopropyl Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to cGMP guidelines where applicable to support our customers' regulatory requirements in therapeutic and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to reach ambient temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.1%
P31 NMR	Single major peak

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.