Description

4-β-Hydroxycholesterol is a specific oxysterol metabolite of cholesterol, playing a significant role in the study of cholesterol metabolism and homeostasis. This compound is a critical reference standard and research chemical for analytical and biochemical applications. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors.

Application

• Biomarker Research: Used as a reference standard in LC-MS/MS and GC-MS methods to quantify endogenous 4β-hydroxycholesterol levels, a biomarker for CYP3A4/5 enzyme activity in drug metabolism studies.
• Pharmaceutical R&D: Serves as a critical intermediate or analytical standard in the development of drugs targeting cholesterol-related pathways, such as those for hypercholesterolemia or liver diseases.
• Diagnostic Kit Manufacturing: Employed in the calibration and validation of diagnostic assays and kits designed to measure oxysterols for clinical research purposes.
• Metabolic Pathway Analysis: Essential for in vitro and in vivo studies investigating the enzymatic conversion of cholesterol to its hydroxycholesterol derivatives and their biological effects.
• Academic Research: Used in universities and research institutes for fundamental studies on atherosclerosis, neurobiology, and the role of oxysterols in cell signaling and inflammation.
• Quality Control: Acts as a high-purity standard for the identity and purity testing of related compounds in pharmaceutical quality control laboratories.

Basic Information

Product Name	4-β-Hydroxycholesterol
CAS No.	17320-10-4
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(3β)-Cholest-5-ene-3,4-diol; 4β-Hydroxycholesterol; 4β-HC; 4β-Hydroxycholest-5-en-3β-ol; 4β-Hydroxy-5-cholesten-3β-ol; 4β-OHC; 4β-Hydroxycholest-5-en-3-ol
EINECS	Contact for details

Quality Control

Our 4-β-Hydroxycholesterol is produced and handled under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. For long-term storage, keep the container sealed in a cool, dry, and well-ventilated area. Allow the product to reach room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.