Description

Phosphate Buffer Solution CAS NO 12111-21-6 is a precisely formulated aqueous solution of monobasic and dibasic phosphate salts, designed to maintain a stable and reliable pH environment. This ready-to-use buffer is critical for ensuring the accuracy and reproducibility of analytical procedures and biological assays across research and industrial settings. It is an essential reagent for laboratories and quality control units in the pharmaceutical, biotechnology, food & beverage, and environmental monitoring sectors.

Application

• Analytical Chemistry: As a mobile phase component and calibration standard in High-Performance Liquid Chromatography (HPLC), Ion Chromatography (IC), and Capillary Electrophoresis (CE).
• Life Science Research: For cell culture media preparation, protein purification, enzyme kinetics studies, and as a diluent in immunoassays like ELISA.
• Pharmaceutical Quality Control: Used in dissolution testing, stability studies, and as a buffering agent in formulation development to maintain product pH.
• Clinical Diagnostics: Serves as a key component in buffer systems for automated clinical analyzers and in-vitro diagnostic (IVD) kits.
• Microbiology: For preparing culture media and as a washing buffer in microbial sample processing.
• Environmental Analysis: Employed in the calibration of pH meters and as a matrix for testing water and soil samples.

Basic Information

Product Name	Phosphate Buffer Solution
CAS No.	12111-21-6
Molecular Formula	Mixture (typically KH₂PO₄ / K₂HPO₄ or NaH₂PO₄ / Na₂HPO₄)
Molecular Weight	N/A (Aqueous Solution)
Synonyms	Phosphate Buffered Saline (PBS); PBS Buffer; Phosphate Buffer; Sörensen's Phosphate Buffer; Dulbecco's PBS; Potassium Phosphate Buffer; Sodium Phosphate Buffer; Inorganic Phosphate Buffer
EINECS	Contact for details

Quality Control

Our Phosphate Buffer Solution is manufactured under a strict quality management system. Each batch is tested to ensure compliance with critical parameters including pH, conductivity, osmolarity, absorbance, and sterility (where applicable). Certificates of Analysis (COA) detailing all test results are provided and available upon request. We support standards including ISO, GMP, and can tailor specifications to meet USP/EP or other pharmacopeial requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) unless specified otherwise for a specialized grade. Do not freeze. For long-term stability of certain formulations, storage at 2-8°C may be recommended. Refer to the specific product label or COA for precise storage instructions.

Specification

Item	Specification
Appearance	Clear, colorless solution
pH @ 25°C	As specified (e.g., 6.8, 7.2, 7.4 ± 0.1)
Conductivity	Within specified range
Osmolality	Within specified range
Absorbance (260 nm / 280 nm)	≤ specified limit
Endotoxin (for sterile grades)	< 0.25 EU/mL or as specified
Sterility (for sterile grades)	Passes test (Membrane Filtration)
Biological Testing (as required)	Passes test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.