Description

Folin And Ciocalteu'S Phenol Reagent is a specialized chemical mixture primarily composed of phosphomolybdic and phosphotungstic acids, used for the colorimetric determination of phenolic compounds and polyphenols. Its core value lies in its high sensitivity and specificity for the Folin-Ciocalteu assay, a cornerstone method for quantifying total phenolic content and antioxidant capacity. This reagent is essential for quality control and research laboratories in the pharmaceutical, nutraceutical, food & beverage, and academic research sectors, where accurate analysis of bioactive compounds is critical.

Application

• Total Phenolic Content (TPC) Analysis: Primary reagent for the standardized Folin-Ciocalteu assay in food, plant extracts, and herbal products.
• Antioxidant Capacity Measurement: Used to evaluate the reducing power of samples, correlating to antioxidant activity in nutritional and cosmetic research.
• Pharmaceutical Quality Control: Assaying phenolic active pharmaceutical ingredients (APIs) and raw materials derived from botanical sources.
• Beverage Industry Testing: Routine analysis of polyphenol levels in wines, beers, teas, and fruit juices.
• Biochemical & Life Science Research: Quantifying phenols in biological samples, cell culture media, and enzymatic reaction products.
• Cosmetic Ingredient Analysis: Testing natural extracts used in skincare formulations for their phenolic antioxidant content.
• Environmental Monitoring: Potential use in detecting phenolic pollutants in water samples.
• Method Development & Validation: Serves as a reference standard for developing and validating new analytical protocols.

Basic Information

Product Name	Folin And Ciocalteu'S Phenol Reagent
CAS No.	12111-13-6
Molecular Formula	H3[P(W3O10)4] / H3[P(Mo3O10)4] (Mixed Acids)
Molecular Weight	Contact for details
Synonyms	Folin-Ciocalteu Reagent; Folin's Reagent; Folin & Ciocalteu's Phenol Reagent; Phosphomolybdic-Phosphotungstic Acid Reagent; FC Reagent; Folin-Denis Reagent (related); Phenol Reagent; Total Phenol Reagent; Gallic Acid Equivalents (GAE) Assay Reagent
EINECS	Contact for details

Quality Control

Our Folin And Ciocalteu'S Phenol Reagent is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure consistent performance, sensitivity, and low blank values critical for reproducible assay results. We provide comprehensive Certificates of Analysis (COA) with key performance metrics upon request, supporting method validation and regulatory compliance in GLP/GMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The reagent is light-sensitive and contains strong acid components; ensure proper ventilation in storage areas and keep away from incompatible materials such as strong bases and reducing agents.

Specification

Item	Specification
Appearance	Yellow to amber-colored solution
Identification (IR/Spectrophotometry)	Conforms
Assay (Titration)	≥ 98.0% (as total acid)
Sensitivity Test (vs. Gallic Acid)	Conforms to reference curve
Absorbance of Reagent Blank	≤ 0.05 (at 765 nm)
Heavy Metals (as Pb)	≤ 10 ppm
Chloride (Cl)	≤ 50 ppm
Sulfate (SO4)	≤ 100 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.