Description

Isopropyl((R)-(((2R,3R,4R,5R)-4-chloro-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxy-4-methyltetrahydrofuran-2-yl)methoxy)(phenoxy)phosphoryl)-D-alaninate is a sophisticated nucleoside phosphoramidite derivative, a critical building block in modern oligonucleotide synthesis. This compound matters because it enables the precise, automated assembly of DNA and RNA sequences, a foundational technology for research, diagnostics, and therapeutic development. It is essential for pharmaceutical R&D teams, biotechnology companies, and academic laboratories engaged in antisense therapy, siRNA development, PCR primers, and genetic analysis.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Development: For the research and production of therapeutic oligonucleotides targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: A crucial reagent in creating short interfering RNA and microRNA for gene silencing studies and therapeutics.
• PCR Primer and Probe Manufacture: Used to synthesize high-purity primers and fluorescent probes for diagnostic and research qPCR applications.
• Genetic Research Tools: For constructing custom oligonucleotides used in sequencing, cloning, mutagenesis, and hybridization.
• Aptamer Development: In the synthesis of nucleic acid aptamers for use as molecular recognition elements in sensors and therapeutics.

Basic Information

Product Name	Isopropyl((R)-(((2R,3R,4R,5R)-4-chloro-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxy-4-methyltetrahydrofuran-2-yl)methoxy)(phenoxy)phosphoryl)-D-alaninate
CAS No.	1496551-77-9
Molecular Formula	C22H27ClN3O9P
Molecular Weight	543.89 g/mol
Synonyms	D-Alanine, N-[isopropyloxy(phenoxy)phosphinyl]-, (2R,3R,4R,5R)-4-chloro-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxy-4-methyltetrahydrofuran-2-yl methyl ester; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Uridine Phosphoramidite Derivative; Nucleoside Phosphoramidite Monomer; DNA/RNA Synthesis Reagent; Antisense Building Block; Oligonucleotide Synthesis Intermediate
EINECS	Contact for details

Quality Control

Our high-purity phosphoramidite monomers are manufactured under strict quality control in a controlled environment to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analysis including HPLC purity assay, ³¹P NMR and ¹H NMR for structural confirmation, and moisture content (Karl Fischer) testing. A detailed Certificate of Analysis (COA) documenting identity, purity, and suitability for automated synthesis is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be brought to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, keep the original sealed vial frozen.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (³¹P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (Karl Fischer)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.