Description

Bafilomycin D is a potent and specific macrolide antibiotic belonging to the bafilomycin family, known for its role as a highly selective inhibitor of vacuolar-type H+-ATPase (V-ATPase). This compound is a critical research tool for studying intracellular vesicle trafficking, autophagy, and lysosomal function. It is primarily utilized by researchers and developers in the life sciences, pharmaceutical, and biotechnology sectors for biochemical research, target validation, and drug discovery programs.

Application

• Biochemical Research: A key reagent for studying V-ATPase activity, lysosomal acidification, and intracellular pH regulation in cell biology experiments.
• Autophagy Studies: Used to inhibit autophagosome-lysosome fusion, making it essential for investigating autophagy pathways and related diseases.
• Pharmaceutical Development: Serves as a reference standard and a lead compound in the discovery of novel therapeutics targeting cancer, osteoporosis, and viral infections where V-ATPase plays a role.
• Microbiology: Employed in research exploring its antibiotic properties against specific fungi and bacteria.
• Neuroscience Research: Applied in studies of neurotransmitter storage and release mechanisms within synaptic vesicles.
• Cell Signaling Pathways: Utilized to dissect signaling cascades affected by lysosomal dysfunction and pH homeostasis.

Basic Information

Product Name	Bafilomycin D
CAS No.	98813-13-9
Molecular Formula	C₄₄H₆₈O₁₄
Molecular Weight	821.0 g/mol
Synonyms	Bafilomycin D; BafD; Concanamycin B; Antibiotic X 14547A; V-ATPase Inhibitor Bafilomycin D; (3S,6S,7R,10R,11S,12S,13R,16S,19R,20S,21S,24S,25S,28R,29S,30S,33R,36S,37R,40S)-37-[(2R,3S,4S,5S,6R)-4-(Dimethylamino)-3-hydroxy-6-methyl-5-(methylamino)oxan-2-yl]oxy-6,11,20,25,29-pentahydroxy-3-[(2R,4S,5S,6S)-5-hydroxy-4-methoxy-6-methyl-4-(2-methylpropanoyloxy)oxan-2-yl]oxy-10,13,16,21,24,30,33,40-octamethyl-1,4,8,15,18,23,26,32,35,39-decaoxaheptacyclo[31.5.2.1²,⁵.¹⁴,¹⁷.²²,³¹.²⁷,³⁶.³⁴,³⁸]dotetracontane-41,42-dione
EINECS	Contact for details

Quality Control

Our Bafilomycin D is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific biological activity assays. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Biological Activity (IC₅₀)	Reported (nM range)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.