Description

Sfi I CAS NO 91930-79-9 is a specialized biochemical reagent, a restriction enzyme widely utilized in molecular biology research and genetic engineering workflows. Its primary value lies in its specific DNA cleavage activity, enabling precise DNA manipulation and analysis, which is critical for advancing scientific discovery and biotechnological applications. This product is essential for research laboratories, diagnostic kit manufacturers, and biopharmaceutical companies involved in genomics, cloning, and nucleic acid analysis.

Application

• Molecular Cloning: Used for the precise digestion of DNA fragments to be inserted into vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: A key tool for genetic fingerprinting and mutation detection.
• Genome Mapping: Facilitates the construction of physical maps of DNA sequences.
• Diagnostic Assay Development: Employed in the design and production of kits for genetic testing and pathogen identification.
• Recombinant DNA Technology: Critical for gene splicing and the production of recombinant proteins.
• Quality Control in Biomanufacturing: Used to verify plasmid identity and integrity during upstream bioprocessing.

Basic Information

Product Name	Sfi I
CAS No.	91930-79-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	SfiI; Endodeoxyribonuclease SfiI; Restriction enzyme Sfi I; Type II restriction enzyme SfiI; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Sfi I is produced and tested under stringent quality management systems to ensure high purity, specific activity, and absence of non-specific nucleases. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing activity units, purity assessments, and stability data. We adhere to relevant quality standards for research-grade enzymes to guarantee reproducible and reliable performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity. For long-term storage, keep aliquots in a non-frost-free freezer.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves specific DNA recognition sequence
Specific Activity	≥ XX,XXX units/mg protein
Purity (SDS-PAGE)	≥ 95%
Non-Specific Nuclease Activity	Not detected
Endonuclease Activity	Absent
Storage Buffer	Tris-HCl, KCl, DTT, Glycerol, EDTA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.