Description

2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carboxy Ethylhydrazine is a stable nitroxide radical derivative, a crucial building block in the synthesis of spin labels and probes for Electron Paramagnetic Resonance (EPR) spectroscopy. Its primary value lies in enabling the study of molecular dynamics, structure, and interactions in complex biological and chemical systems. This compound is essential for researchers and manufacturers in the fields of biophysics, structural biology, and advanced materials science who require high-purity, functionalized spin labels.

Application

• Spin Labeling for EPR/ESR Spectroscopy: Covalently attached to biomolecules (proteins, nucleic acids, lipids) to probe local environment, mobility, and distances.
• Site-Directed Spin Labeling (SDSL): A key reagent for introducing nitroxide radicals at specific sites in proteins to study folding, conformational changes, and protein-protein interactions.
• Polymer and Materials Science: Used as a radical probe to investigate polymer chain dynamics, phase separation, and the microstructure of advanced materials.
• Oxidation/Reduction Studies: Serves as a stable radical standard or redox-active moiety in mechanistic studies of electron transfer processes.
• Bioconjugation and Probe Synthesis: The reactive hydrazine and carboxyl groups allow for versatile conjugation to antibodies, enzymes, or other targeting molecules to create functional EPR probes.
• Antioxidant Research: Utilized as a model compound to study radical scavenging activity and the mechanisms of action of antioxidants.

Basic Information

Product Name	2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carboxy Ethylhydrazine
CAS No.	89367-58-8
Molecular Formula	C12H22N3O3
Molecular Weight	256.33 g/mol
Synonyms	3-Carboxy-PROXYL Hydrazide; 3-Carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl Hydrazide; 3-Carboxy-PROXYL hydrazine; 3-Carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy, hydrazide; PROXYL-3-carboxylic acid hydrazide; TEMPO-3-carboxylic acid hydrazide; 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxylic acid ethylhydrazine
EINECS	Contact for details

Quality Control

Our 2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carboxy Ethylhydrazine is manufactured under strict quality systems to ensure batch-to-batch consistency and high radical purity, which is critical for sensitive EPR applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as assay (by HPLC or EPR), radical purity, and impurity profile. We support research and industrial applications requiring reliable and traceable spin label precursors.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and degradation of the nitroxide radical. For long-term storage, desiccate at -20°C. The container should be kept in a dry, well-ventilated area away from incompatible materials.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Radical Purity (EPR)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.