Description

Bafilomycin B1 CAS NO 88899-56-3 is a potent and specific macrolide antibiotic belonging to the bafilomycin family, functioning as a highly selective inhibitor of vacuolar-type H+-ATPase (V-ATPase). This compound is a critical research tool for studying intracellular vesicle trafficking, autophagy, and lysosomal function. It is essential for researchers and developers in the fields of cell biology, pharmacology, and biochemistry, particularly for investigating cancer mechanisms, neurodegenerative diseases, and novel therapeutic pathways.

Application

• Biomedical Research: A fundamental tool for studying autophagy, lysosomal acidification, and intracellular protein degradation pathways in cell culture models.
• Pharmacology & Drug Discovery: Used as a reference standard and a molecular probe to screen for and validate new V-ATPase inhibitors and autophagy modulators.
• Cancer Research: Employed to investigate the role of V-ATPase in tumor cell survival, metastasis, and drug resistance mechanisms.
• Neuroscience Studies: Applied in research on neurodegenerative diseases like Alzheimer's and Parkinson's to understand lysosomal dysfunction.
• Microbiology: Used in studies of bacterial and fungal physiology due to its antibiotic properties against certain Gram-positive bacteria.
• Biochemical Assay Development: Serves as a key control compound in developing high-throughput screening assays for V-ATPase activity.

Basic Information

Product Name	Bafilomycin B1
CAS No.	88899-56-3
Molecular Formula	C₃₅H₅₈O₉
Molecular Weight	622.83 g/mol
Synonyms	Bafilomycin B1; Bafilomycin B 1; Antibiotic Bafilomycin B1; Baf B1; (3S,6S,7R,10R,11S,12S,13R,16S,19S,22R,23E,25S,26R,27S,28R,29S,30S,33S,34R,35S,36R,37S,38R)-34-[(2R,3S,4S,5S,6R)-4-Amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-6,10,12,16,22,26,30,33,35,37-decamethyl-2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39-octatriacontaoxacyclooctatriacontane-1,5,11,15,19,25,29,33,37-nonanone
EINECS	Contact for details

Quality Control

Our Bafilomycin B1 is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications such as assay purity, related substances, and residual solvents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Purity (HPLC, Area%)	≥ 98.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.