Description

3-Chloro-3-Benzyldiazirine is a specialized diazirine-based photoaffinity labeling reagent, a critical tool for probing biomolecular interactions and mapping binding sites. Its core value lies in generating highly reactive carbene intermediates upon photolysis, enabling covalent cross-linking to target molecules with high spatial and temporal control. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require precise tools for studying protein-ligand interactions, enzyme mechanisms, and complex biological pathways.

Application

• Photoaffinity Labeling (PAL): A primary reagent for covalent cross-linking in the study of protein-small molecule, protein-protein, and protein-nucleic acid interactions.
• Chemical Biology & Proteomics: Used to identify and characterize unknown binding sites, map protein surfaces, and discover new drug targets.
• Bioconjugation & Probe Development: Serves as a key building block for synthesizing advanced molecular probes and bifunctional cross-linkers.
• Materials Science Research: Applied in surface modification and polymer chemistry to create functionalized materials via photochemical grafting.
• Mechanistic Studies: Employed to trap and analyze transient reaction intermediates in catalytic and biochemical processes.

Basic Information

Product Name	3-Chloro-3-Benzyldiazirine
CAS No.	88211-05-6
Molecular Formula	C8H7ClN2
Molecular Weight	166.61 g/mol
Synonyms	3-Chloro-3-phenyldiaziridine; 3-Chloro-3-phenyl diaziridine; 3-Chloro-3-phenyldiazirine; 3-Phenyl-3-chlorodiazirine; Benzene, (3-chloro-3H-diazirin-3-yl)-; (3-Chloro-3H-diazirin-3-yl)benzene; Photoaffinity Labeling Reagent; PAL Reagent
EINECS	Contact for details

Quality Control

Every batch of 3-Chloro-3-Benzyldiazirine is manufactured and tested under strict quality protocols to ensure high purity and consistent performance for sensitive research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to confirm identity, purity, and stability. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at recommended temperatures between -20°C to -10°C to maintain stability. The container should be kept in a freezer and allowed to equilibrate to room temperature before opening to minimize moisture ingress and thermal stress. Handle and store under an inert atmosphere for long-term stability.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid or low-melting solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Total Impurities	≤ 5.0%
Solubility	Soluble in common organic solvents (e.g., DCM, THF, Acetone)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.