Description

Polybuffer 74 is a high-purity, amphoteric buffer substance specifically formulated for chromatographic applications. This product is essential for creating precise, linear pH gradients in chromatofocusing, a powerful technique for separating proteins and other biomolecules based on their isoelectric points. It is a critical reagent for researchers and process developers in the biotechnology and pharmaceutical industries who require high-resolution separation and purification of sensitive biological compounds. Polybuffer 74 CAS NO 82658-86-4 offers excellent buffering capacity and reproducibility for demanding analytical and preparative workflows.

Application

• Chromatofocusing: Primary application for generating linear pH gradients in column chromatography for the separation of proteins, peptides, and enzymes.
• Biomolecule Purification: Used in the purification and isolation of antibodies, recombinant proteins, and other biological macromolecules in downstream bioprocessing.
• Analytical Biochemistry: Serves as a key component in high-resolution analytical methods for characterizing protein charge heterogeneity and isoform distribution.
• Isoelectric Focusing (IEF): Can be utilized as a carrier ampholyte alternative or supplement in certain IEF protocols for improved separation.
• Process Development: Employed in R&D and scale-up laboratories to optimize purification protocols for therapeutic proteins and vaccines.
• Enzyme Studies: Useful in experiments investigating enzyme activity and stability across a defined pH range.

Basic Information

Product Name	Polybuffer 74
CAS No.	82658-86-4
Molecular Formula	Proprietary mixture of buffering components
Molecular Weight	N/A (Polymeric buffer mixture)
Synonyms	Polybuffer® 74; Polybuffer 74 (Pharmacia); PB 74; Ampholyte Buffer 74; Chromatofocusing Buffer 74; Polybuffers; Carrier Ampholyte 74; Polyampholyte Buffer
EINECS	Contact for details

Quality Control

Our Polybuffer 74 is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance reliability for critical separations. Each lot is tested to confirm its buffering profile, conductivity, and suitability for chromatofocusing applications. While not a single chemical entity, quality is assured through rigorous functional testing. A Certificate of Analysis (COA) detailing specific performance characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Keep the container tightly sealed when not in use to prevent absorption of atmospheric carbon dioxide, which can alter the buffering characteristics.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow liquid
pH Range (Working)	pH 4 to 7 (for chromatofocusing)
Conductivity	Low (suitable for chromatographic elution)
Buffering Capacity	High and consistent across the pH range
UV Absorbance	Low at 280 nm and 254 nm
Solubility	Completely miscible with water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.