Description

Acetonitrile With 0.1% Ammonium Acetate is a high-purity, ready-to-use mobile phase additive specifically formulated for liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS). This pre-mixed solution is critical for ensuring consistent chromatographic performance, enhancing peak shape, and improving ionization efficiency in sensitive analytical applications. It is an essential reagent for analytical laboratories in the pharmaceutical, biotechnology, and environmental testing sectors where reproducibility and data integrity are paramount.

Application

• HPLC & UHPLC Mobile Phase: Used as a key organic solvent component in reversed-phase chromatography, often paired with aqueous buffers for the separation of a wide range of compounds.
• LC-MS Analysis: The addition of 0.1% ammonium acetate provides a volatile buffer system that enhances ionization in electrospray (ESI) and atmospheric pressure chemical ionization (APCI) sources, improving sensitivity and signal stability.
• Pharmaceutical Quality Control (QC): Employed in the assay and impurity profiling of active pharmaceutical ingredients (APIs) and finished drug products to meet pharmacopeial standards (e.g., USP, EP).
• Biomolecule Analysis: Suitable for the separation and analysis of peptides, proteins, oligonucleotides, and other polar biomolecules in research and development.
• Environmental Testing: Applied in the detection and quantification of pesticides, herbicides, and other organic contaminants in water and soil samples.
• Forensic and Clinical Toxicology: Used in methods for screening and confirming drugs of abuse, metabolites, and other small molecules in biological matrices.

Basic Information

Product Name	Acetonitrile With 0.1% Ammonium Acetate
CAS No.	148642-19-7
Molecular Formula	C2H3N • C2H7NO2 (Mixture)
Molecular Weight	41.05 (Acetonitrile) / 77.08 (Ammonium Acetate)
Synonyms	Acetonitrile + 0.1% Ammonium Acetate; Acetonitrile/Ammonium Acetate Solution; LC-MS Grade Acetonitrile with Ammonium Acetate; HPLC Grade Acetonitrile with Buffer; Methyl Cyanide with Ammonium Acetate; MeCN with NH4OAc; Acetonitrile, mixture with ammonium acetate; Mobile Phase Additive for LC-MS.
EINECS	200-835-2 (Acetonitrile) / 211-162-9 (Ammonium Acetate)

Quality Control

Our Acetonitrile With 0.1% Ammonium Acetate is manufactured under strict quality management systems. Each batch is tested to ensure it meets stringent specifications for HPLC and LC-MS suitability, with critical parameters including UV cutoff, absorbance, fluorescence, and particle count. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a well-ventilated area. Keep away from strong acids and oxidizing agents. Due to the volatile and flammable nature of acetonitrile, all storage areas should comply with local regulations for flammable solvents.

Specification

Item	Specification
Appearance	Clear, colorless liquid
Identification (IR)	Conforms
Assay (HPLC)	Acetonitrile: ≥99.9% Ammonium Acetate: 0.10% ± 0.02%
UV Absorbance (HPLC Grade)	See COA for lot-specific data
Water (Karl Fischer)	≤0.05%
Non-Volatile Residue	≤5 ppm
Particle Count	Meets or exceeds USP <788> requirements

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.