Description

Pvu I is a high-purity restriction endonuclease enzyme, a critical tool for precise DNA manipulation in molecular biology research and biotechnological applications. Its primary value lies in its specific recognition and cleavage of the palindromic sequence 5'-CGATCG-3', enabling researchers to perform accurate genetic analysis, cloning, and sequencing. This product is essential for professionals in pharmaceutical R&D, academic research institutions, and biotechnology companies engaged in genetic engineering and diagnostic assay development.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into plasmid vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used for genetic fingerprinting, mutation detection, and genotyping studies.
• DNA Sequencing Library Preparation: Facilitates the fragmentation and preparation of genomic DNA for next-generation sequencing platforms.
• Recombinant DNA Technology: A fundamental tool for constructing recombinant DNA molecules in genetic engineering workflows.
• Diagnostic Assay Development: Employed in the design and validation of molecular diagnostic tests for pathogens or genetic disorders.
• Gene Mapping and Analysis: Critical for physical mapping of genomes and analyzing gene structure.

Basic Information

Product Name	Pvu I
CAS No.	81295-33-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Endodeoxyribonuclease PvuI; PvuI Restriction Enzyme; Restriction Endonuclease Pvu I; Type II Site-Specific Deoxyribonuclease PvuI; EC 3.1.21.4; PvuI (from Proteus vulgaris); CGATCG-specific Endonuclease
EINECS	Contact for details

Quality Control

Our Pvu I is manufactured and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot undergoes rigorous testing for specific activity, purity (free from non-specific nucleases and phosphatases), and fidelity. Certificates of Analysis (COA) detailing activity units, storage buffer composition, and quality control parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. The product is supplied in a stabilized storage buffer; handle on ice when in use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves λ DNA to produce expected fragment pattern
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	Single band corresponding to ~32 kDa subunit
Non-Specific Nuclease Activity	Not detectable
Phosphatase Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.