Description

3-Hydroxyphenyl-3H-Diazirine is a specialized heterocyclic compound featuring a photoreactive diazirine group, making it a valuable chemical tool for photoaffinity labeling and crosslinking studies. Its core value lies in its ability to form covalent bonds with target biomolecules upon UV light activation, enabling the study of protein-protein interactions, ligand-receptor binding, and other complex biological processes. This compound is essential for researchers and developers in the fields of chemical biology, proteomics, and pharmaceutical discovery who require high-purity, reliable photoactivatable probes.

Application

• Photoaffinity Labeling (PAL): A critical reagent for identifying and characterizing molecular targets of bioactive compounds, drugs, and natural products.
• Bioconjugation & Crosslinking: Used to create stable covalent links between proteins, nucleic acids, and other biomolecules for structural and functional analysis.
• Proteomics Research: Enables the mapping of protein interaction networks and the identification of transient binding sites.
• Drug Discovery: Facilitates target identification and validation for novel therapeutic agents by capturing drug-target complexes.
• Materials Science: Serves as a surface modification agent for creating functionalized polymers and materials with specific biological activity.
• Chemical Probe Synthesis: A key building block for synthesizing advanced molecular probes used in live-cell imaging and diagnostic assays.

Basic Information

Product Name	3-Hydroxyphenyl-3H-Diazirine
CAS No.	80863-10-1
Molecular Formula	C7H6N2O
Molecular Weight	134.14 g/mol
Synonyms	3-(3H-Diazirin-3-yl)phenol; m-Hydroxyphenyldiazirine; 3-Hydroxybenzenediaziridine; 3-(Diazirin-3-yl)phenol; Phenol, 3-(3H-diazirin-3-yl)-; Photoaffinity Labeling Reagent; Diazirine-based Crosslinker
EINECS	Contact for details

Quality Control

Our 3-Hydroxyphenyl-3H-Diazirine is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods (NMR, IR) for structural confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes. We adhere to cGMP principles where applicable to support our clients in regulated research and development environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its light-sensitive nature, handling should be performed under subdued light or amber glass to maintain stability and potency. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen to prevent potential degradation.

Specification

Item	Specification
Appearance	Off-white to light yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.