Description

Phorbol 12,20-Diacetate is a specific, high-purity derivative of the phorbol diterpene family, a class of compounds known for their potent biological activity. This compound is a critical reference standard and research chemical for studying protein kinase C (PKC) signaling pathways and tumor promotion mechanisms. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on oncology, dermatology, and cell biology.

Application

• Biomedical Research: As a potent activator of protein kinase C (PKC), it is used extensively in cell signaling studies, apoptosis research, and mechanistic investigations of tumor promotion.
• Pharmacology & Toxicology: Serves as a key reference standard in the development and validation of assays for PKC activity and in toxicological studies of phorbol esters.
• Oncology Research: Employed in laboratory research to model and study the molecular mechanisms of cancer development and progression.
• Dermatology Research: Used in studies related to skin inflammation and hyperproliferative disorders due to its effects on cellular differentiation and proliferation.
• Biochemical Reagent: Acts as a high-purity biochemical tool for modulating PKC-dependent pathways in in vitro and ex vivo experimental systems.
• Quality Control & Standardization: Provides a certified reference material for the calibration of analytical instruments and the quality control of related pharmaceutical or research products.

Basic Information

Product Name	Phorbol 12,20-Diacetate
CAS No.	77573-11-6
Molecular Formula	C28H38O9
Molecular Weight	518.60 g/mol
Synonyms	12,20-Diacetoxyphorbol; 12-O-Acetylphorbol 20-acetate; Phorbol-12,20-diacetate; 4,9,12β,13,20-Pentahydroxy-1,6-tigliadien-3-one 12,20-diacetate; NSC 326223; 12β-Acetoxy-20-acetoxyphorbol; TPA-related compound (12,20-Diacetate)
EINECS	Contact for details

Quality Control

Our Phorbol 12,20-Diacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.