Description

Aponatamycin is a naturally derived macrolide antibiotic with potent activity against Gram-positive bacteria and select mycobacterial strains. It serves as a critical research tool in antimicrobial resistance studies, mode-of-action investigations, and novel antibiotic development programs. Pharmaceutical R&D laboratories, academic microbiology departments, and contract research organizations (CROs) rely on high-purity Aponatamycin CAS NO 60395-06-4 for reproducible biological assays and structure–activity relationship (SAR) profiling.

Application

• Antibiotic mechanism-of-action studies targeting bacterial ribosomal subunits
• In vitro screening of novel macrolide analogues for enhanced stability or spectrum
• Reference standard in HPLC and LC-MS/MS quantification methods for antibiotic residue analysis
• Tool compound in mycobacterial growth inhibition assays (e.g., against Mycobacterium smegmatis)
• Cell-based assays evaluating efflux pump inhibition and resistance modulation
• Preclinical pharmacokinetic and metabolism studies (ADME)
• Quality control reference material for fermentation-derived macrolide batches
• Teaching and training applications in advanced medicinal chemistry and microbiology laboratories

Basic Information

Product Name	Aponatamycin
CAS No.	60395-06-4
Molecular Formula	C38H63NO12
Molecular Weight	725.91 g/mol
Synonyms	Aponatamycin; NSC 321994; Antibiotic A-131; A-131; Aponatamycin A; Aponatamycin I; 3-O-(2,6-Dideoxy-3-C-methyl-L-ribo-hexopyranosyl)-3-dehydro-3,4-didehydro-erythromycin A; 3-O-Desosaminyl-3-dehydro-3,4-didehydro-erythromycin A
EINECS	Not listed (no EINECS assigned)

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches meet stringent purity criteria for research-grade antibiotics, including identity confirmation by HPLC retention time matching, FT-IR spectral verification, and assay purity ≥98.5% (HPLC area %). Testing adheres to principles aligned with USP General Chapter <1058> Analytical Instrument Qualification and ISO/IEC 17025 guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (nitrogen or argon) to prevent degradation. Due to its hygroscopic nature, maintain low humidity conditions (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Individual impurities ≤0.5%; Total impurities ≤1.5%
Residual Solvents (GC)	Meets ICH Q3C limits
Water Content (Karl Fischer)	≤2.0%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.