Description

3-[3-(Bromomethyl)-5-Iodophenyl]-3-(Trifluoromethyl)-3H-Diazirine is a highly specialized, photoactivatable crosslinking agent belonging to the diazirine class of compounds. This molecule is engineered for advanced biochemical research, enabling the covalent labeling and capture of transient biomolecular interactions upon exposure to ultraviolet light. It is an essential tool for researchers in pharmaceutical development, proteomics, and structural biology who require precise, irreversible photoaffinity labeling. The compound's unique trifluoromethyl-diazirine and bifunctional aryl halide structure provides exceptional reactivity and versatility for probe synthesis.

Application

• Photoaffinity Labeling (PAL): A critical reagent for covalently tagging drug targets, receptors, and enzymes to study binding sites and interaction mechanisms.
• Chemical Biology Probe Synthesis: Serves as a key building block for creating bifunctional crosslinkers and activity-based probes (ABPs) for proteomic profiling.
• Proteomics and Interactome Mapping: Used to capture and identify protein-protein interactions and ligand-protein complexes in live cells or complex biological mixtures.
• Structural Biology Studies: Facilitates the stabilization of weak complexes for analysis via techniques like X-ray crystallography or cryo-electron microscopy.
• Drug Discovery & Target Identification: Enables the elucidation of mechanisms of action for novel therapeutic compounds by identifying their direct molecular targets.
• Materials Science Surface Modification: Applied in the functionalization of surfaces and nanomaterials for biosensor development through photo-induced covalent attachment.

Basic Information

Product Name	3-[3-(Bromomethyl)-5-Iodophenyl]-3-(Trifluoromethyl)-3H-Diazirine
CAS No.	309250-32-6
Molecular Formula	C10H5BrF3IN2
Molecular Weight	400.96 g/mol
Synonyms	3-(3-(Bromomethyl)-5-iodophenyl)-3-(trifluoromethyl)-3H-diazirine; 3-(3-Bromomethyl-5-iodophenyl)-3-(trifluoromethyl)diazirine; 3-(3-Bromomethyl-5-iodophenyl)-3-(trifluoromethyl)-3H-diazirine; TPD-Br,I; Trifluoromethylphenyl Diazirine Bromo-Iodo Derivative; Photoaffinity Crosslinker 309250-32-6; (3-(Bromomethyl)-5-iodophenyl)(trifluoromethyl)diazirine
EINECS	Contact for details

Quality Control

Supplied as a high-purity research-grade chemical, 3-[3-(Bromomethyl)-5-Iodophenyl]-3-(Trifluoromethyl)-3H-Diazirine undergoes rigorous analytical characterization to ensure batch-to-batch consistency and performance reliability. Our quality assurance includes comprehensive testing via HPLC, NMR (1H, 13C, 19F), and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for sensitive biochemical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dedicated freezer. The compound is light-sensitive and should be handled under subdued light. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep away from strong acids and organic solvents.

Specification

Item	Specification
Appearance	White to off-white crystalline solid or powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile, THF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.