Description

3-Chloro-3-(4-Nitrophenyl)-3H-Diazirine is a specialized photoaffinity labeling reagent, a key tool in chemical biology and pharmaceutical research. Its core value lies in its ability to generate highly reactive carbenes upon photolysis, enabling the covalent cross-linking and identification of biomolecular interaction sites. This compound is essential for researchers and developers in proteomics, drug discovery, and materials science who require precise molecular mapping and target identification.

Application

• Photoaffinity Labeling (PAL): A critical reagent for mapping protein-ligand, protein-protein, and protein-nucleic acid interactions in drug discovery and structural biology.
• Proteomics Research: Used to identify and characterize low-abundance protein targets and transient binding sites that are difficult to capture with traditional methods.
• Bioconjugation and Probe Development: Serves as a cross-linker for creating stable, covalently bonded bioconjugates, fluorescent probes, and affinity tags.
• Materials Surface Functionalization: Applied in surface science to modify polymers and other materials with specific bioactive molecules for sensor or implant development.
• Mechanistic Studies in Enzymology: Employed to trap and study enzyme-substrate complexes and catalytic intermediates.

Basic Information

Product Name	3-Chloro-3-(4-Nitrophenyl)-3H-Diazirine
CAS No.	39184-67-3
Molecular Formula	C7H4ClN3O2
Molecular Weight	197.58 g/mol
Synonyms	3-Chloro-3-(4-nitrophenyl)diazirine; 4-Nitrophenylchlorodiazirine; 3-Chloro-3-(p-nitrophenyl)-3H-diazirine; 3H-Diazirine, 3-chloro-3-(4-nitrophenyl)-; p-Nitrophenylchlorodiazirine; 3-Chloro-3-(4-nitrophenyl)diaziridine; Photoaffinity Labeling Reagent 39184-67-3
EINECS	Contact for details

Quality Control

Our 3-Chloro-3-(4-Nitrophenyl)-3H-Diazirine is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We support research and development with material traceability and documentation in compliance with ISO 9001 quality management principles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is light-sensitive (store away from light) and should be handled under inert atmosphere when appropriate to maximize shelf life. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	< 0.5%
Solubility	Soluble in common organic solvents (DMSO, DMF, Acetone)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.