Description

3-Chloro-3-(4-Methylphenyl)-3H-Diazirine is a specialized diazirine-based photoaffinity labeling reagent, a critical tool in modern chemical biology and pharmaceutical research. Its core value lies in its ability to form highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and identification of biomolecular interaction sites. This compound is essential for researchers and developers in proteomics, drug discovery, and materials science who require precise molecular mapping and target identification.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalently tagging proteins, nucleic acids, and other biological targets to study binding sites and molecular interactions.
• Proteomics and Drug Discovery: Used to identify unknown protein targets of bioactive small molecules and to validate drug-target engagement in complex biological systems.
• Chemical Biology Probes: Incorporated into probe molecules for activity-based protein profiling (ABPP) and mapping enzyme active sites.
• Materials Science Research: Employed in surface functionalization and polymer cross-linking studies where controlled, light-initiated covalent bonding is required.
• Bioconjugation: Facilitates the stable attachment of ligands, dyes, or other molecules to biomolecules for diagnostic and therapeutic development.

Basic Information

Product Name	3-Chloro-3-(4-Methylphenyl)-3H-Diazirine
CAS No.	39184-65-1
Molecular Formula	C8H7ClN2
Molecular Weight	166.61 g/mol
Synonyms	3-Chloro-3-(p-tolyl)diazirine; 3-Chloro-3-(4-methylphenyl)diazirine; 3-(4-Methylphenyl)-3-chlorodiazirine; p-Tolyl-3-chlorodiazirine; Diazirine, 3-chloro-3-(4-methylphenyl)-; Photoaffinity Labeling Reagent; PAL Reagent
EINECS	Contact for details

Quality Control

Every batch of 3-Chloro-3-(4-Methylphenyl)-3H-Diazirine is manufactured and tested under strict quality protocols to ensure high purity and performance consistency for sensitive research applications. Our products undergo rigorous quality testing, including chromatographic purity analysis and spectroscopic identification, to ensure compliance with exacting R&D standards. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is light-sensitive (store away from light). Allow the container to reach ambient temperature before opening to minimize moisture condensation. Keep under an inert atmosphere for long-term storage to maximize stability.

Specification

Item	Specification
Appearance	Yellow to orange liquid or low-melting solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Related Substances (HPLC)	≤ 5.0% total

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.