Description

3H-Diazirine, 3-(2-Bromoethyl)-3-(3-Butyn-1-Yl)- is a highly functionalized diazirine derivative, a specialized class of carbene precursors used in photoaffinity labeling and cross-linking applications. Its unique bifunctional structure, featuring both a bromoethyl and a butynyl group, makes it an invaluable tool for the site-specific modification of biomolecules and materials under mild, UV-light activation. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, proteomics, and materials science who require precise, covalent attachment points for probes, tags, or linkers.

Application

• Photoaffinity Labeling (PAL): A critical reagent for mapping drug-protein and protein-protein interaction sites by forming irreversible cross-links upon UV irradiation.
• Chemical Biology Probes: Used to synthesize bifunctional cross-linkers for studying biological structures and dynamics in live cells.
• Bioconjugation & Protein Engineering: Enables the site-specific attachment of fluorophores, affinity tags (e.g., biotin), or other functional moieties to proteins and antibodies.
• Polymer & Surface Functionalization: Serves as a photo-activatable handle for modifying polymer backbones or material surfaces to introduce new chemical functionalities.
• Proteomics Research: Employed in activity-based protein profiling (ABPP) and target identification strategies for novel therapeutics.
• Development of Diagnostic Kits: Integral in creating advanced assay components that require stable, covalent immobilization of capture molecules.

Basic Information

Product Name	3H-Diazirine, 3-(2-Bromoethyl)-3-(3-Butyn-1-Yl)-
CAS No.	2432830-88-9
Molecular Formula	C7H9BrN2
Molecular Weight	201.06 g/mol
Synonyms	3-(2-Bromoethyl)-3-(but-3-yn-1-yl)-3H-diazirine; 3-(2-Bromoethyl)-3-(3-butynyl)diazirine; Bifunctional Diazirine Crosslinker; Photo-activatable Bromo-Alkyne Crosslinker; (2-Bromoethyl)(3-butynyl)diazirine; Diazirine-Br-Alkyne; PAL Reagent with Bromo and Alkyne Handles
EINECS	Contact for details

Quality Control

Every batch of our high-value diazirine compounds is synthesized and purified under controlled conditions to ensure exceptional consistency and performance. We employ advanced analytical techniques, including NMR, HPLC, and LC-MS, to verify chemical identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. Store away from strong oxidizers and bases.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid or low-melting solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.