Description

2-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Ethan-1-Amine Hydrochloride is a specialized, high-purity chemical building block featuring a diazirine photoaffinity label and an alkyne handle for bioorthogonal click chemistry. This dual-functional compound is essential for advanced proteomics research, enabling the covalent capture and identification of transient protein-ligand interactions. It is primarily utilized by pharmaceutical R&D teams and academic research institutions focused on drug discovery and chemical biology.

Application

• Photoaffinity Labeling (PAL): A critical reagent for mapping small molecule binding sites on target proteins through UV-induced cross-linking.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme families and their inhibitors.
• Target Identification & Validation: Facilitates the discovery of novel biological targets for therapeutic compounds in early-stage drug development.
• Bioconjugation & Probe Synthesis: The alkyne moiety allows for efficient conjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) to create fluorescent or affinity tags.
• Structural Biology Studies: Aids in elucidating protein structure and function by providing covalent probes for mass spectrometry and crystallography.
• High-Throughput Screening (HTS): Serves as a key intermediate in developing screening assays for fragment-based drug discovery.

Basic Information

Product Name	2-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Ethan-1-Amine Hydrochloride
CAS No.	2089291-69-8
Molecular Formula	C8H12N3Cl
Molecular Weight	185.65 g/mol
Synonyms	2-(3-(But-3-yn-1-yl)-3H-diazirin-3-yl)ethan-1-amine hydrochloride; 3-(2-Aminoethyl)-3-(but-3-yn-1-yl)diaziridine hydrochloride; Diazirine Alkyne Amine Hydrochloride; Photoaffinity Labeling Reagent with Alkyne; PAL-Alkyne Crosslinker HCl; Clickable Diazirine Probe; Bioorthogonal Photoaffinity Probe; 2089291-69-8
EINECS	Contact for details

Quality Control

Our 2-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Ethan-1-Amine Hydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, critical for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to relevant industry standards for research-grade chemicals, ensuring reliable performance in your proteomics and chemical biology workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic (moisture-sensitive) nature, the container must be sealed under dry conditions and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.