Description

[2-[2-[2-[2-Formyl-5-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Phenoxy]Ethoxy]Ethoxy]Ethyl]Carbamic Acid 1,1-Dimethylethyl Ester is a sophisticated, heterobifunctional crosslinking reagent featuring a photoreactive diazirine group and a protected amine. This compound is engineered to enable the covalent capture of transient biomolecular interactions, a critical function in advanced proteomics and chemical biology research. It is an essential tool for researchers and developers in pharmaceutical discovery, structural biology, and the development of novel diagnostic platforms.

Application

• Photoaffinity Labeling (PAL): A key reagent for identifying and mapping low-affinity protein-ligand binding sites and protein-protein interaction interfaces.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to discover and validate novel drug targets by covalently tagging active enzymes.
• Bioconjugation & Probe Development: Serves as a versatile linker for synthesizing affinity probes, imaging agents, and antibody-drug conjugates (ADCs).
• Structural Biology: Facilitates the study of membrane protein structures and dynamics by crosslinking interacting subunits for analysis.
• Drug Discovery: Employed in fragment-based drug discovery to identify and optimize lead compounds by stabilizing their binding to target proteins.
• Material Science: Used to functionalize surfaces and nanomaterials with bioactive molecules for biosensor and diagnostic device development.

Basic Information

Product Name	[2-[2-[2-[2-Formyl-5-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Phenoxy]Ethoxy]Ethoxy]Ethyl]Carbamic Acid 1,1-Dimethylethyl Ester
CAS No.	299931-13-8
Molecular Formula	C21H28F3N3O6
Molecular Weight	475.46 g/mol
Synonyms	Boc-PEG4-Benzaldehyde-Diazirine; 3-(Trifluoromethyl)-3H-diazirin-3-yl-PEG4-Boc-amine-Benzaldehyde; 4-Formyl-3-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenoxy-PEG4-t-butyl carbamate; Benzaldehyde-PEG4-Boc-Diazirine; Boc-N-amido-PEG4-Benzaldehyde-Diazirine; Photoaffinity Labeling Reagent (PEG4-Boc-Diazirine); t-Butyl N-[2-[2-[2-[2-[3-[3-(trifluoromethyl)-3H-diazirin-3-yl]-4-formylphenoxy]ethoxy]ethoxy]ethoxy]ethyl]carbamate
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to stringent quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.