Description

3-Chloro-3-Isopropyl-3H-Diazirine is a highly specialized, light-sensitive diazirine compound used as a critical photoaffinity labeling reagent. Its core value lies in its ability to generate highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and study of biomolecular interactions. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require precise tools for mapping protein-ligand binding sites and understanding complex biological mechanisms.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent tagging of biological targets (e.g., proteins, nucleic acids) upon UV light activation to study binding sites and interactions.
• Chemical Biology & Proteomics Research: Used to create probes for identifying and validating drug targets, understanding metabolic pathways, and profiling enzyme activity.
• Pharmaceutical R&D: Integral in early-stage drug discovery for mapping ligand-receptor interactions and aiding in the design of new therapeutic agents.
• Bioconjugation & Probe Synthesis: Serves as a versatile building block for synthesizing photoactivatable cross-linkers and molecular probes.
• Materials Science: Potential use in surface functionalization and the creation of photo-responsive polymeric materials.

Basic Information

Product Name	3-Chloro-3-Isopropyl-3H-Diazirine
CAS No.	29648-80-4
Molecular Formula	C5H9ClN2
Molecular Weight	132.59 g/mol
Synonyms	3-Chloro-3-(propan-2-yl)diazirine; 3-Isopropyl-3-chlorodiazirine; 3H-Diazirine, 3-chloro-3-(1-methylethyl)-; Diaziridine, 3-chloro-3-isopropyl-; Photoaffinity Labeling Reagent; PAL Reagent; Isopropyldiazirine Chloride
EINECS	Contact for details

Quality Control

Every batch of 3-Chloro-3-Isopropyl-3H-Diazirine is manufactured and tested under strict quality protocols to ensure high purity and performance consistency for research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and GC-MS analysis, to verify identity, purity, and stability. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at recommended temperatures (typically 2-8°C or as specified). Due to its light-sensitive and highly volatile nature, minimize exposure to light and handle in a fume hood. Keep away from heat, sparks, and open flames.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.