Description

3-Methyl-3H-diazirine-3-propionic acid methyl ester is a specialized diazirine-based photoaffinity labeling reagent. This compound is valued for its ability to generate highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and study of biomolecular interactions. It is primarily utilized by researchers and manufacturers in the life sciences sector for applications in chemical biology, proteomics, and drug discovery. The product is supplied to meet the stringent purity requirements of advanced research and development.

Application

• Photoaffinity Labeling (PAL): A critical reagent for mapping protein-ligand binding sites, protein-protein interactions, and other biological interfaces through covalent cross-linking initiated by UV light.
• Chemical Biology Probes: Used in the synthesis of activity-based probes (ABPs) and bifunctional cross-linkers to study enzyme mechanisms and metabolic pathways.
• Proteomics Research: Facilitates the identification and characterization of low-abundance proteins or transient complexes by creating stable, covalent bonds for subsequent isolation and analysis.
• Drug Discovery: Employed in target identification and validation studies to elucidate the molecular targets of bioactive compounds.
• Material Science: Potential use in surface functionalization and polymer chemistry to create light-responsive materials and coatings.
• Bioconjugation: Serves as a versatile handle for attaching various tags (e.g., fluorophores, biotin) to biomolecules for detection and purification.

Basic Information

Product Name	3-Methyl-3H-Diazirine-3-Propionic Acid Methyl Ester
CAS No.	25055-91-8
Molecular Formula	C6H8N2O2
Molecular Weight	140.14 g/mol
Synonyms	Methyl 3-(3-Methyl-3H-diazirin-3-yl)propanoate; 3-[3-Methyl-3H-diazirin-3-yl]propionic Acid Methyl Ester; Diazirine-Propionate Methyl Ester; Photoaffinity Labeling Reagent; PAL Reagent; Methyl Diazirinylpropionate; 3-Methyl-3H-diazirinepropanoic Acid Methyl Ester
EINECS	Contact for details

Quality Control

Every batch of 3-Methyl-3H-diazirine-3-propionic acid methyl ester is produced and tested under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Our products undergo rigorous quality testing, including chromatographic purity analysis and structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its light-sensitive nature, handling should be performed under subdued light or amber glass to prevent premature decomposition. Keep the container tightly sealed when not in use to protect from moisture.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.