Description

3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoic Acid is a specialized diazirine-based photoaffinity labeling reagent, a critical tool in chemical biology and proteomics research. Its core value lies in its ability to form highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and identification of biomolecular interaction sites. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and structural biology who require precise mapping of protein-ligand and protein-protein interactions.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent capture of transient or weak biomolecular interactions in drug target identification and mechanism-of-action studies.
• Chemical Proteomics: Used to profile protein function and discover small-molecule binding sites within complex biological samples.
• Bioconjugation & Probe Synthesis: Serves as a versatile building block for synthesizing activity-based probes (ABPs) and bifunctional cross-linkers.
• Structural Biology: Facilitates the study of protein structures and dynamics by trapping interaction complexes for subsequent analysis via mass spectrometry or crystallography.
• Drug Discovery Platforms: Integral to high-throughput screening platforms designed to identify and validate novel therapeutic targets.
• Material Science Surface Modification: Potential application in creating functionalized surfaces with photoreactive groups for biosensor development.

Basic Information

Product Name	3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoic Acid
CAS No.	1450754-37-6
Molecular Formula	C9H10N2O2
Molecular Weight	178.19 g/mol
Synonyms	3-[3-(But-3-yn-1-yl)-3H-diazirin-3-yl]propanoic acid; 3-(3-But-3-ynyl-3H-diazirin-3-yl)propionic Acid; Diazirine-Butynyl-Propionic Acid; Photoaffinity Labeling Reagent 1450754-37-6; PAL Reagent with Alkyne Handle; Carbene Precursor Reagent; Alkyne-functionalized Diazirine Acid
EINECS	Contact for details

Quality Control

Our 3-(3-(But-3-Yn-1-Yl)-3H-Diazirin-3-Yl)Propanoic Acid is manufactured under strict quality protocols to ensure the high purity and reactivity required for sensitive research applications. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to confirm identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee traceability and compliance with your research standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. The container should be kept in a cool, dry, and well-ventilated area. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.