Description

3-Hydroxy Detomidine-15N2,D2 Hydrochloride is a stable, isotopically labeled analog of the veterinary sedative detomidine, featuring dual nitrogen-15 and deuterium-2 incorporation at defined positions for enhanced mass spectrometric detection and quantification. This compound serves as a critical internal standard in quantitative bioanalysis—enabling high-precision LC-MS/MS measurement of detomidine and its metabolites in biological matrices with minimized matrix effects and instrument variability. It is essential for pharmaceutical R&D laboratories, contract research organizations (CROs), and regulatory bioanalytical labs conducting ADME, toxicokinetic, and residue studies in large-animal and equine models.

Application

• Internal standard for LC-MS/MS quantification of detomidine in plasma, urine, and tissue homogenates
• Bioanalytical method development and validation per FDA/EMA bioanalytical guidelines
• Pharmacokinetic and residue depletion studies in veterinary drug registration dossiers
• Metabolite identification and pathway mapping in equine and bovine liver microsome assays
• Quality control of detomidine-based veterinary formulations via isotopic dilution calibration
• Reference material for certified reference laboratories (CRLs) producing ISO 17034-compliant standards
• Stable isotope tracer studies investigating α₂-adrenergic receptor binding kinetics
• Calibration standard for multi-residue veterinary drug monitoring programs (e.g., EU SANTE/11945/2015)

Basic Information

Product Name	3-Hydroxy Detomidine-15N2,D2 Hydrochloride
CAS No.	1346603-61-9
Molecular Formula	C₁₀H₁₃D₂¹⁵N₂O·HCl
Molecular Weight	248.72 g/mol (calculated for monohydrochloride salt)
Synonyms	3-Hydroxy-detomidine-¹⁵N₂,D₂ hydrochloride; 3-Hydroxy-4-(2,3-dihydro-1H-imidazol-2-yl)-2,2-dimethyl-N-(¹⁵N₂,D₂)-propylbenzenamine hydrochloride; [¹⁵N₂,D₂]-3-Hydroxydetomidine HCl; Detomidine-3-OH-¹⁵N₂,D₂·HCl; 3-Hydroxydetomidine-d₂-¹⁵N₂ hydrochloride; NSC 759287-¹⁵N₂,D₂·HCl; 4-(2,3-Dihydro-1H-imidazol-2-yl)-3-hydroxy-2,2-dimethyl-N-(¹⁵N₂,D₂)-propylbenzenamine hydrochloride
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for isotopic enrichment (>98% ¹⁵N at both nitrogens, >99% D at designated positions), chemical purity (≥98.5% by HPLC-UV), identity (IR, NMR, HRMS), and absence of residual solvents (ICH Q3C compliant). Testing follows USP & EP general chapters for reference standards and adheres to ISO/IEC 17025 accredited laboratory practices.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccated environment before opening.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HRMS)	m/z matches calculated [M+H]⁺ for C₁₀H₁₃D₂¹⁵N₂O·HCl
Assay (HPLC-UV)	≥98.5%
Isotopic Enrichment (¹⁵N)	>98% at both nitrogen positions (by ¹⁵N NMR)
Isotopic Enrichment (D)	>99% at designated carbon-bound deuterium positions (by ²H NMR)
Related Substances (HPLC)	Any single impurity ≤0.3%; Total impurities ≤1.0%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤1.0% w/w

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.