Description

n-(4-Aminobenzenesulfonyl) Sulfamethoxazole-D4 is a deuterated stable isotope-labeled analog of sulfamethoxazole, specifically designed for use as an internal standard in quantitative bioanalytical assays. It delivers exceptional analytical precision and accuracy in LC-MS/MS applications due to its matched physicochemical behavior and isotopic purity. This compound is essential for pharmaceutical R&D laboratories, contract research organizations (CROs), and regulatory bioequivalence studies requiring rigorous pharmacokinetic and metabolic profiling.

Application

• Internal standard for LC-MS/MS quantification of sulfamethoxazole in human plasma, serum, and tissue homogenates
• Bioanalytical method development and validation for clinical pharmacology and toxicokinetic studies
• Reference material in regulatory submissions to FDA, EMA, and PMDA for generic drug bioequivalence testing
• Quality control in pharmaceutical manufacturing for sulfonamide antibiotic stability and degradation pathway analysis
• Metabolite identification and reaction phenotyping studies involving cytochrome P450 enzymes (e.g., CYP2C9)
• Stable isotope dilution assays (SIDA) in forensic toxicology and doping control laboratories
• Calibration standard preparation for high-sensitivity therapeutic drug monitoring (TDM) workflows
• Reference compound in inter-laboratory proficiency testing programs for antimicrobial residue analysis

Basic Information

Product Name	n-(4-Aminobenzenesulfonyl) Sulfamethoxazole-D4
CAS No.	1346603-52-8
Molecular Formula	C15H13D4N4O4S2
Molecular Weight	392.44 g/mol
Synonyms	Sulfamethoxazole-d4 internal standard; N-(4-Aminobenzenesulfonyl)-sulfamethoxazole-d4; SMX-d4 sulfonamide conjugate; [D4]-Sulfamethoxazole sulfonamide adduct; 4-Aminobenzenesulfonyl-sulfamethoxazole-d4; Sulfamethoxazole-N-(4-aminobenzenesulfonyl)-d4; SMX-ABSA-d4; N4-(4-Aminobenzenesulfonyl)sulfamethoxazole-d4
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for isotopic enrichment (>98% D-incorporation at designated positions), chemical purity (≥99.0% by HPLC-UV), identity (IR and NMR confirmation), and absence of critical impurities (e.g., residual solvents, heavy metals). The material is manufactured under ISO 9001-certified processes and complies with ICH Q7 guidelines for reference standards used in pharmaceutical analysis.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to reference 1H and 2H NMR data
Assay (HPLC-UV)	≥99.0%
Isotopic Purity (MS)	≥98% D-incorporation at labeled positions
Residual Solvents (GC)	Meets ICH Q3C limits
Water Content (Karl Fischer)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.