Description

Diazo Biotin-Peg3-Azide is a multifunctional, heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound integrates a biotin affinity tag, a polyethylene glycol (PEG3) spacer for improved solubility and reduced steric hindrance, a photoactivatable diazirine group for UV-induced covalent crosslinking, and an azide moiety for copper-free click chemistry. It is essential for researchers and developers in life sciences and chemical biology who require precise, stable, and efficient labeling of biomolecules for detection, pull-down assays, and proteomic studies. Its unique structure enables versatile, site-specific conjugation strategies critical for drug discovery, diagnostic assay development, and biomaterial functionalization.

Application

• Proteomics & Interactomics: Photoaffinity labeling for the identification and mapping of protein-protein interactions, ligand-receptor binding sites, and transient complexes.
• Bioconjugation & Probe Development: Site-specific labeling of antibodies, peptides, oligonucleotides, and other biomolecules with biotin for subsequent detection with streptavidin conjugates.
• Chemical Biology & Target Identification: As a critical tool in activity-based protein profiling (ABPP) to discover and validate novel drug targets by crosslinking active site residues.
• Diagnostic Assay Development: Functionalization of surfaces (e.g., chips, beads) and biosensors to create highly specific capture and detection platforms.
• Material Science: Surface modification of polymers and nanomaterials to introduce bio-recognition elements for targeted delivery systems.
• Pull-down & Enrichment Strategies: Immobilization of bait molecules onto solid supports (e.g., streptavidin beads) for the selective isolation and purification of target complexes from complex mixtures.

Basic Information

Product Name	Diazo Biotin-Peg3-Azide
CAS No.	1339202-33-3
Molecular Formula	C₂₈H₄₄N₁₀O₇S
Molecular Weight	664.78 g/mol
Synonyms	Biotin-PEG3-Diazirine-Azide; Biotin-PEG3-Photo-Azide; Diazirine-PEG3-Biotin-Azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(14-azido-3,6,9,12-tetraoxatetradecyl)-2-(3-(3-methyl-3H-diazirin-3-yl)propanamido)pentanamide; Photoactivatable Biotin-PEG3-N3; Heterobifunctional Crosslinker Biotin-Diazirine-PEG3-Azide; Biotinylated Diazirine Crosslinker
EINECS	Contact for details

Quality Control

Our Diazo Biotin-Peg3-Azide is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment, MS (Mass Spectrometry) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake and decomposition.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.