Description

4-((4-(Dimethylamino)Phenyl)Azo)Benzoic Acid, Succinimidyl Ester is a highly reactive, amine-reactive heterobifunctional crosslinking agent and labeling reagent. This compound is critical for creating stable amide bonds with primary amines, enabling the conjugation of biomolecules such as proteins, peptides, and antibodies with various probes, surfaces, or other molecules. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, diagnostics development, and advanced materials science, where precise and efficient covalent attachment is required.

Application

• Bioconjugation and Protein Labeling: Primary reagent for covalently attaching fluorescent dyes, enzymes (e.g., HRP), biotin, or other functional groups to antibodies, proteins, and peptides for immunoassays (ELISA, Western Blot) and diagnostic kits.
• Diagnostic Probe Synthesis: Used in the development of sensitive and specific detection probes for clinical diagnostics and biomedical research.
• Surface Functionalization: Key intermediate for modifying nanoparticles, microspheres, biosensor chips, and other solid supports with amine-reactive groups to create active surfaces for biomolecule immobilization.
• Drug Delivery System Development: Employed in the research and fabrication of antibody-drug conjugates (ADCs) and other targeted therapeutic delivery platforms.
• Affinity Chromatography Ligand Coupling: Facilitates the attachment of specific ligands (e.g., proteins, peptides) to chromatography resins for purification processes.
• Polymer and Biomaterial Modification: Enables the chemical modification of polymers and hydrogels to introduce bioactive motifs or alter material properties for tissue engineering and biomedical applications.

Basic Information

Product Name	4-((4-(Dimethylamino)Phenyl)Azo)Benzoic Acid, Succinimidyl Ester
CAS No.	146998-31-4
Molecular Formula	C19H18N4O4
Molecular Weight	366.37 g/mol
Synonyms	NHS-DABCYL; DABCYL NHS Ester; 4-((4-(Dimethylamino)phenyl)azo)benzoic acid N-hydroxysuccinimide ester; DABCYL acid, succinimidyl ester; DABCYL-SE; N-Succinimidyl 4-((4-(dimethylamino)phenyl)azo)benzoate; 4-Dimethylaminoazobenzene-4'-carboxylic acid, N-succinimidyl ester; DABCYL-X, SE
EINECS	Contact for details

Quality Control

Our 4-((4-(Dimethylamino)Phenyl)Azo)Benzoic Acid, Succinimidyl Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive bioconjugation applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); desiccants are recommended for long-term storage. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple crystalline powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.