Description

2,4-Dinitrophenol-Lysine-Fluorescein Conjugate is a sophisticated, triple-functionalized chemical probe designed for advanced biochemical research and diagnostics. This compound matters for its unique ability to combine the quenching properties of dinitrophenol, the linker functionality of lysine, and the fluorescent signaling of fluorescein in a single molecule. It is primarily needed by researchers and manufacturers in the fields of immunoassay development, fluorescence resonance energy transfer (FRET) studies, and high-sensitivity diagnostic kit production.

Application

• Immunoassay Development: Used as a hapten-fluorophore conjugate for creating competitive fluorescence immunoassays (FIA) and enzyme-linked immunosorbent assays (ELISA).
• FRET-Based Probes: Serves as a critical component in the design of molecular beacons and FRET pairs for real-time monitoring of enzymatic activity or biomolecular interactions.
• Diagnostic Reagents: Incorporated into lateral flow assays and other point-of-care diagnostic devices for the detection of specific antibodies, hormones, or small molecule analytes.
• Cell Labeling & Imaging: Functions as a versatile tag for labeling proteins, antibodies, or other biomolecules for fluorescence microscopy and flow cytometry.
• Bioconjugation Research: Acts as a model compound for studying and optimizing conjugation chemistry between haptens, linkers, and reporter molecules.
• Quality Control in Biopharma: Employed as a reference standard or tracer in the quality control of therapeutic monoclonal antibodies and other biologics.

Basic Information

Product Name	2,4-Dinitrophenol-Lysine-Fluorescein Conjugate
CAS No.	134649-45-9
Molecular Formula	C33H27N5O11
Molecular Weight	669.60 g/mol
Synonyms	DNP-Lys-FITC; Dinitrophenyl-Lysine-Fluorescein Isothiocyanate Conjugate; 2,4-DNP-ε-Lys-FITC; Fluorescein Isothiocyanate labeled DNP-Lysine; Hapten-Fluorophore Conjugate; DNP hapten-FITC tracer; FDNP-Lys-FITC; [5-(2,4-Dinitrophenylamino)-5-oxopentyl]amino-fluorescein conjugate
EINECS	Contact for details

Quality Control

Every batch of our 2,4-Dinitrophenol-Lysine-Fluorescein Conjugate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility. Our quality protocols are designed to meet the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will lead to degradation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Extinction Coefficient	Provided on COA
Fluorescence Properties	Provided on COA
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.