Description

Naphthol As-Mx Phosphate CAS NO 1596-56-1 is a specialized biochemical reagent primarily used as a substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic detection is required. It is essential for laboratories in the life sciences, clinical diagnostics, and pharmaceutical development sectors.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A key chromogenic substrate for detecting alkaline phosphatase conjugates in immunoassays.
• Histochemistry & Immunohistochemistry (IHC): Used for visualizing antigen localization in tissue sections.
• Western Blotting: Employed as a detection reagent for alkaline phosphatase-labeled antibodies.
• Clinical Diagnostics: Integral to automated analyzers for measuring alkaline phosphatase levels in serum.
• Molecular Biology Research: Serves as a substrate in nucleic acid hybridization detection systems.
• Pharmaceutical Quality Control: Used in assays to test for enzymatic activity in biopharmaceutical products.

Basic Information

Product Name	Naphthol As-Mx Phosphate
CAS No.	1596-56-1
Molecular Formula	C18H15NO6P
Molecular Weight	372.29 g/mol
Synonyms	3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate; Naphthol AS-MX phosphate; 3-Hydroxy-4'-methyl-2-naphthanilide phosphate; AS-MX Phosphate; Naphthol AS-MX phosphoric acid ester; Fast Red TR Salt substrate; Alkaline Phosphatase Substrate; 2-Naphthol AS-MX phosphate; Naphthol AS-MX phosphate salt
EINECS	Contact for details

Quality Control

Our Naphthol As-Mx Phosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is tested for identity, purity, and suitability for use as an enzymatic substrate. A comprehensive Certificate of Analysis (COA) detailing purity (typically >98% by HPLC), absorbance profile, and functional performance is provided with every shipment to guarantee it meets the stringent requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Test	Suitable for use as Alkaline Phosphatase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.