Description

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate is a high-purity chromogenic substrate widely used in enzymatic assays. Its primary value lies in its sensitivity and reliability as a reagent for detecting peroxidase activity, producing a distinct blue colorimetric signal. This compound is essential for research, diagnostic, and quality control laboratories in the biotechnology, pharmaceutical, and clinical diagnostics industries.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A critical chromogenic substrate for horseradish peroxidase (HRP) in both research and clinical diagnostic kits.
• Immunohistochemistry (IHC): Used for visualizing antigen-antibody complexes in tissue sections.
• Western Blotting: Employed as a detection reagent for HRP-conjugated secondary antibodies.
• Biosensor Development: Serves as a key component in electrochemical and colorimetric biosensors for analyte detection.
• Diagnostic Test Manufacturing: A raw material for in-vitro diagnostic (IVD) devices, including point-of-care tests.
• Food Safety & Environmental Testing: Used in assays to detect pathogens, toxins, or specific contaminants.
• Research & Development: A fundamental tool in biochemical and molecular biology research for quantifying enzyme activity.

Basic Information

Product Name	3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate
CAS No.	207738-08-7
Molecular Formula	C16H22N2·2HCl·xH2O
Molecular Weight	Anhydrous: 317.27 g/mol (Free base)
Synonyms	TMB Dihydrochloride Hydrate; 3,3',5,5'-Tetramethylbenzidine 2HCl Hydrate; TMB-2HCl·H2O; TMB Substrate; TMB Chromogen; Benzidine, 3,3',5,5'-tetramethyl-, dihydrochloride, hydrate; TMB for ELISA; TMB Salt
EINECS	Contact for details

Quality Control

Our 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for diagnostic and research use. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in water and DMSO
Absorbance (in solution)	Meets performance criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.